30,803 results

1. KaiC

   Plasmid

   #41884

   Depositor

   Michael Rust

   Article

   Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

   Insert

   Kai C
   

   Tags

   histadine

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gi-c009

   Plasmid

   #173079

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   GNAI1 (GNAI1 Human)
   

   Tags

   mCitrine

   Expression

   Bacterial

   Mutation

   full length, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV-2-NSP3-c002

   Plasmid

   #173084

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
   

   Expression

   Bacterial

   Mutation

   E1084-E1192, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HSP90B1

   Plasmid

   #39076

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSP90B1 (HSP90B1 Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCoofy22

   Plasmid

   #43992

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His10 and S

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6p-hDcp2

   Plasmid

   #72216

   Purpose

   bacterial expression of GST-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F146Y and E198K (please see depositor's comm…

   Promoter

   tac

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBEST-pTar-UTR1-TetR-T500

   Plasmid

   #45780

   Depositor

   Richard Murray, Vincent Noireaux

   Insert

   Tetracycline repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTar

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. p405MET3

   Plasmid

   #15962

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   MET3 promoter is between SacI and XbaI restrictio…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. LOVS1K

   Plasmid

   #31981

   Purpose

   Expresses a LOVS1K, a light-controllable synthetic protein to modulate local or global Ca2+ signaling

   Depositor

   Kevin Truong

   Article

   Pham et al Chem Biol. 2011 Jul 29;18(7):880-90.

   Insert

   LOVS1K
   

   Tags

   RFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV, p10

   Available Since

   Dec. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pHH0103_PLCG1_SH3

    Plasmid

    #110035

    Purpose

    Protein expression and purification of PLCG1_SH3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PLCG1_SH3 (PLCG1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbS8k-RFP_E83K

    Plasmid

    #109412

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    E83K repA mutation to increase copy number

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Stat1-SH2

    Plasmid

    #46511

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat1 (STAT1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 488-683

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis8-4b-SaDAH

    Plasmid

    #140128

    Purpose

    Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Sinomenium acutum
    

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Endophilin Full

    Plasmid

    #47408

    Depositor

    Peter McPherson

    Article

    Micheva et al J Biol Chem. 1997 Oct 24;272(43):27239-4

    Insert

    Endophilin
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

15. H14-MBP-SUMO-FLAG-LEM

    Plasmid

    #101778

    Purpose

    Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    MAN1 (LEMD3 Human)
    

    Tags

    His14-MBP-SUMO-FLAG

    Expression

    Bacterial

    Mutation

    amino acids 6-51 of human MAN1 (LEM domain)

    Promoter

    T5

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Lyn-SH2

    Plasmid

    #46452

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Lyn (LYN Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 106-213

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATAD2B

    Plasmid

    #39046

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ATAD2B (ATAD2B Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. SPIN2B (SPIN2BA-c011)

    Plasmid

    #98238

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN2BA (SPIN2B Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHybr_r2a>m2a(silent)-srt-his

    Plasmid

    #124807

    Purpose

    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2a (Fc silent)
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    L234A/L235A/N297A

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24a MDH_STRAW

    Plasmid

    #204240

    Purpose

    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_STRAW (mdh Streptomyces avermitilis)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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