32,069 results
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Plasmid#32181DepositorInsertTALE Monomer NG
ExpressionBacterialAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-S-4P
Plasmid#165094Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
ExpressionBacterialAvailable SinceMarch 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBW213ara-hrpS
Plasmid#61435PurposepBAD18-cm encoding rbs33-hrpS-terDepositorInsertrbs33-hrpS-ter
ExpressionBacterialPromoterpBADAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB226
Plasmid#170016PurposeEncodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Defensin HNP-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS
Plasmid#154229PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
S5_EcoRV_Ef1a_AscI
Plasmid#128469PurposeEf1a promoterDepositorInsertEf1a promoter
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTara:500*
Plasmid#107954PurposeArabinose-inducible T7*RNAPDepositorInsertT7RNAP (modified)
UseSynthetic BiologyExpressionBacterialMutationR632SAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneTagsHis10 Tag - 3C cleavage siteExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-mRFP
Plasmid#128126PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterpBen (benzoate responsive promoter)Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1171
Plasmid#119253Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1170
Plasmid#119252Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
Tags6xhis-tag and MBPExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCOLA kiwi sensor sfGFP
Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNMT3B
Plasmid#25163PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOD3-mClover-nat
Plasmid#120328PurposeBacterial mClover promoter trapDepositorInsertmClover
ExpressionBacterialMutationD103N (see comments below)Promoter(None)Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)
Plasmid#165489PurposeXenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activityDepositorInsert2xLyn-ERex-Venus-bPAC(F198Y)
UseCdna expression, xenopus oocyteTagsVenusExpressionBacterialPromoterT7Available SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (1-120)
Plasmid#62065PurposeExpression of human MDM2 (1-120) in e. coliDepositorInsertMDM4 (MDM4 Human)
TagsGSTExpressionBacterialMutationcontains residues 1-120PromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only