30,803 results

1. pGEX PLCg1(C)-SH2

   Plasmid

   #46469

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(C)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 656-766

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEW4: pET-32a Dot1L(2-386)

   Plasmid

   #124099

   Purpose

   Dot1L(2-386) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-386) (DOT1L Human)
   

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTU425

   Plasmid

   #178984

   Purpose

   pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   TPR-CHAT H585A C627A
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFTK036

   Plasmid

   #171308

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   amdS ANIA_08777 acetamidase, selection marker
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. DT042A

   Plasmid

   #159403

   Purpose

   Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.T5-LacO-6xHis-sfGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T5 promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SLK_HUMAN_D0

   Plasmid

   #79733

   Purpose

   This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SLK (SLK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. RGD-sfGFP-I27-CC

   Plasmid

   #85392

   Purpose

   MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling

   Depositor

   Khalid Salaita

   Article

   Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

   Insert

   RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB2-mNeptune2

   Plasmid

   #104012

   Purpose

   Plasmid encoding mNeptune2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mNeptune2
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC236

   Plasmid

   #65951

   Purpose

   Medium Repressor plasmid for two-strain system

   Depositor

   Matthew Bennett

   Article

   Chen et al Science. 2015 Aug 28;349(6251):986-9. do

   Insert

   CinI
   

   Tags

   LAA tagged

   Expression

   Bacterial

   Promoter

   Prhl/lac-m

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET mOCR His6 LIC cloning vector (2Oc-T)

    Plasmid

    #37238

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and mOCR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-CARD14-E138A

    Plasmid

    #69921

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    E138A

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. ptetmCherry_prey_plasmid-2

    Plasmid

    #34617

    Depositor

    Lingchong You

    Article

    Song et al Nat Chem Biol. 2009 Dec;5(12):929-35. Ep

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    ptet

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKL13-MgtC bb118

    Plasmid

    #158982

    Purpose

    Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mgtC
    

    Expression

    Bacterial

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PHGDH

    Plasmid

    #38841

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pLpp5oGFP

    Plasmid

    #69345

    Purpose

    Orthogonal GFP message under lpp5 promoter

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    super folder GFP
    

    Expression

    Bacterial

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a+GFP-CAMSAP2 CKK

    Plasmid

    #59032

    Purpose

    expresses CKK domain of CAMSAP2 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CKK domain (CAMSAP2 Human)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

    Plasmid

    #116965

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse TF cDNA

    Plasmid

    #15454

    Depositor

    Nigel Mackman

    Article

    Mackman et al FASEB J. 1995 Jul . 9(10):883-9.

    Insert

    Tissue factor (F3 Mouse)
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pQCascade-IS186

    Plasmid

    #140627

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFR-mTq2-antiFRET-P2A-jRCaMP1b

    Plasmid

    #191468

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jRCaMP1b
    

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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