32,457 results
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Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only
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pUC18T-mini-Tn7T-Gm-REP
Plasmid#64966Purposemini-Tn7 vector with oriR6K, for attTn7 site determinationDepositorInsertoriR6K
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL005
Plasmid#92327PurposeExpress FtsZ tagged with mEos3.2 in E coliDepositorInsertFtsZ
TagsmEos3.2ExpressionBacterialPromoterT5-lacAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-H2B(1-114)_wt
Plasmid#118252PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNaDepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialPromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5084
Plasmid#168179Purposebacteria reporterDepositorInsertPEM7::dsred PmsrA::sfgfp
ExpressionBacterialMutationnoneAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT3037
Plasmid#122544PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW213ara-hrpS
Plasmid#61435PurposepBAD18-cm encoding rbs33-hrpS-terDepositorInsertrbs33-hrpS-ter
ExpressionBacterialPromoterpBADAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
USP21 (3MTN)
Plasmid#28112PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP21 (USP21 Human)
TagsHis tag with Thrombin cleavageExpressionBacterialMutationcatalytic domain only (amino acid residues 209-56…Available SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM33_Nsp7
Plasmid#156476PurposeBacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tagDepositorInsertNsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA183
Plasmid#74247PurposepSTL1-dPSTRr(2,1)DepositorInsertpSTL1-dPSTRr(2,1)
ExpressionBacterial and YeastAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG/E17TAG
Plasmid#68298PurposeS2TAG/E17TAG GFP reporterDepositorInsertGFP S2TAG/E17TAG
TagsHisExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/mNeptune2-nat
Plasmid#120337PurposeBacterial mNeptune2 labelingDepositorInsertmNeptune2
ExpressionBacterialPromoterproDAvailable SinceAug. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO9Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-msGFP2 (C48S C70S)
Plasmid#218956PurposeExpresses proinsulin fused to msGFP2 (C48S C70S) in bacteriaDepositorInsertproinsulin-msGFP2 C48S C70S
TagsproinsulinExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V
Plasmid#159542PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12VDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12VAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only