34,239 results

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pBS SK(+)/mGATA2

Plasmid

#83823

Purpose

mGATA2 cDNA

Depositor

Douglas Engel

Insert

GATA2 (Gata2 Mouse)

Expression

Bacterial

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pHLK

Plasmid

#64182

Purpose

harbours marker genes HIS3, LEU2 and LYS2

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHUK

Plasmid

#64183

Purpose

harbours marker genes HIS3, URA3 and LYS2

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHM

Plasmid

#64173

Purpose

harbours marker genes HIS3 and MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4C (216-233) (pGEX2T(TEV))

Plasmid

#80635

Purpose

Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

Depositor

Wesley Sundquist

Publication

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4C (CHMP4C Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 216-233

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas7/pHMGWA

Plasmid

#81184

Purpose

Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLUM

Plasmid

#64169

Purpose

harbours marker genes LEU2, URA3 and MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:G249A

Plasmid

#79163

Purpose

Expresses inactive WspR G249A under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

G249A

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

Plasmid

#72240

Purpose

Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

GLT1 GAL1p 24xPP7 V3

Expression

Bacterial

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160-GCN4

Plasmid

#74608

Purpose

Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Publication

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, GCN4, Strep II, and superfolder GFP

Expression

Bacterial

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKT3Ts-goldyTALEN

Plasmid

#80330

Purpose

Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

Depositor

Jeffrey Essner

Publication

Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

Type

Empty backbone

Use

TALEN

Expression

Bacterial

Promoter

T3

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNR276

Plasmid

#79577

Purpose

TP901 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR239

Plasmid

#79576

Purpose

A118 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Publication

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Expression

Bacterial

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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MetNI (Lm)

Plasmid

#78316

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Publication

Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

Inserts

MetI
MetN

Tags

6x Histidine Tag and 10x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T-HEPN

Plasmid

#75296

Purpose

Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Publication

Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

Insert

Human Sacsin 4441-4579

Tags

GST-Tag

Expression

Bacterial

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_4

Plasmid

#78208

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

Depositor

Martin Webb

Publication

Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

Insert

matB (His6 C106A R286C T303A Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A R286C T303A Q457C K488A

Promoter

T7

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_3

Plasmid

#78207

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

Depositor

Martin Webb

Publication

Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

Insert

matB (His6 C106A T167A R286C Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A T167A R286C Q457C K488A

Promoter

T7

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJKW 0643

Plasmid

#74121

Purpose

Photinus pyralis luciferin sulfotransferase LST - pHis8-4

Depositor

Jing-Ke Weng

Publication

Fallon et al Biochemistry. 2016 May 26.

Insert

Photinus pyralis firefly luciferin sulfotransferase

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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