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3xFLAG-dCas9/p-bacteria

Plasmid

#64325

Purpose

Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Publication

Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

Insert

3xFLAG-dCas9

Use

CRISPR; Tetracycline-inducible

Tags

3xFLAG

Expression

Bacterial

Mutation

D10A, H840A

Promoter

pLtetO-1

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK001

Plasmid

#65108

Purpose

Part Plasmid Entry Vector to be used in the Dueber YTK system.

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

none

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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Beta-Lactamase

Plasmid

#62729

Purpose

Expresses Beta-Lactamase protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Beta-Lactamase

Tags

His tag

Expression

Bacterial

Mutation

Deletion of periplasmic signal domain

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKK233-hCaM

Plasmid

#47598

Depositor

Emanuel Strehler

Publication

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CaM (CALM3 Human)

Expression

Bacterial

Mutation

contains 469 bp fragment (nt 92-559)

Promoter

trc

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pExodus CMV.Artemis

Plasmid

#40211

Purpose

Mammalian expression of codon-optimized Artemis.

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Artemis (DCLRE1C Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDDYFP-A

Plasmid

#40288

Depositor

Robert Campbell

Publication

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

ddYFP-A

Tags

His

Expression

Bacterial

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pUC8 human IFN-gamma

Plasmid

#17600

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

interferon-gamma cDNA (IFNG Human)

Expression

Bacterial

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pDDRFP-A1

Plasmid

#36289

Depositor

Robert Campbell

Publication

Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

Insert

Dimerization dependent RFP A1

Tags

His tag

Expression

Bacterial

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pET mCitrine LIC cloning vector (u-mCitrine)

Plasmid

#29771

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCitrine

Expression

Bacterial

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GST TopBP1 (aa 32-1522) His

Plasmid

#20375

Depositor

Aziz Sancar

Publication

Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

Insert

TopBP1 (TOPBP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Starts at aa32

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pB1H2w5-Prd

Plasmid

#18039

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

Paired (prd Fly)

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NFAT5 luciferase

Plasmid

#14110

Depositor

Anjana Rao

Publication

Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

Insert

NM_001113178.2 (NFAT5 Human)

Use

Luciferase

Expression

Bacterial

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Publication

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 gamma GST

Plasmid

#13280

Depositor

Michael Yaffe

Publication

Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

Insert

14-3-3 gamma (Ywhag Rat)

Tags

GST

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Publication

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBbS5a-Opto-Cre-Vvd-2

Plasmid

#160400

Purpose

Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd-2

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Publication

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Publication

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Expression

Bacterial

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Publication

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FRESCA-syntide

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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