30,736 results

1. pLG009 (DRT type 1)

   Plasmid

   #157887

   Purpose

   Expresses the DRT type 1 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 1 (RT-nitrilase)
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6p1-HH1713

   Plasmid

   #124994

   Purpose

   E. coli expression of recombinant H. hepaticus antigen HH_1713

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH_1713
   

   Expression

   Bacterial

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGT-S1

   Plasmid

   #122582

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG011 (DRT type 3)

   Plasmid

   #157889

   Purpose

   Expresses the DRT type 3 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 3 (RT + RT)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-ch-TOG-His6

   Plasmid

   #69108

   Purpose

   For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC18-mini-Tn7T-Gm-lux

   Plasmid

   #64963

   Purpose

   mini-Tn7 luxCDABE transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   lux operon
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLG005 (Retron-TIR)

   Plasmid

   #157883

   Purpose

   Expresses the retron-TIR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Retron-TIR
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS_nanorod_105b

   Plasmid

   #120347

   Purpose

   phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Staple sequences for a DNA origami nanorod
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWP78-PVCpnf1-16

    Plasmid

    #198271

    Purpose

    Unmodified pPVC; contains WT PVC structural/accessory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf1-16
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18T-mini-Tn7T-Tp-ecfp

    Plasmid

    #65034

    Purpose

    TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

    Plasmid

    #205022

    Purpose

    mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET-MagLOV

    Plasmid

    #219957

    Purpose

    Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

    Depositor

    Andrew York

    Article

    Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

    Insert

    MagLOV
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    AsLOV2 C450P L496V Q513K G528K D540M

    Promoter

    T7

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBHM2329

    Plasmid

    #171686

    Purpose

    pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    C. neoformans U6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPET-PKR/PPase

    Plasmid

    #42934

    Depositor

    James Cole

    Article

    Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

    Inserts

    protein kinase R (EIF2AK2 Human)
    phage lambda phosphatase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBad-PhoCl2c

    Plasmid

    #164035

    Purpose

    Improved PhoCl variant with enhanced dissociation efficiency

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2c
    

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Promoter

    araBad promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLG007 (Ec86 retron)

    Plasmid

    #157885

    Purpose

    Expresses the Ec86 retron defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Ec86 retron
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pλPR cI-Elysis

    Plasmid

    #176891

    Purpose

    Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

    Depositor

    James Inglese

    Article

    Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

    Insert

    Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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