30,258 results

1. pGEX6p1-HH1713

   Plasmid

   #124994

   Purpose

   E. coli expression of recombinant H. hepaticus antigen HH_1713

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH_1713
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. His 3C MKK6 1-334

   Plasmid

   #87881

   Purpose

   E. coli expression of MKK6 aa 1-334

   Depositor

   Peter Mace

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   aa 1-334

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-His-AlaRS

   Plasmid

   #124103

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Alanyl-tRNA ligase (alaS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQFT-mNG

   Plasmid

   #203675

   Purpose

   pQFT with mNeonGreen under control of PQJ

   Depositor

   Urs Jenal

   Article

   Klotz et al Appl Environ Microbiol. 2023 May 18:e002

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PQJ

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AASDHPPT

   Plasmid

   #39192

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AASDHPPT (AASDHPPT Human)
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. T7-LacO-GFP_ColE1

   Plasmid

   #171648

   Purpose

   Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-LacO-GFPssrA-T7 Stop
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28GSTlinker-LIC

   Plasmid

   #119970

   Purpose

   Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEND-349_Cacnes_ori

    Plasmid

    #225604

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNBU2_erm-TetR-P1T_DP-GH023

    Plasmid

    #90324

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1-Pls3-sfGFP

    Plasmid

    #205750

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pls3 (Phyperspank mutant, IPTG inducible)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIP4_2A4D

    Plasmid

    #234196

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2A4D (UBE2V1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pNIC28-MacroGreen

    Plasmid

    #160665

    Purpose

    Expresses GFP fusion of a multisite mutant of the Af1521 protein

    Depositor

    Herwig Schüler

    Article

    Garcia-Saura et al Commun Biol. 2021 Jul 28;4(1):919. doi:

    Insert

    Af1521 modified synthetic construct eGFP fusion
    

    Use

    Tags

    eGFP and hexahistidine_TEV recognition

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Sfp

    Plasmid

    #159617

    Purpose

    Expression plasmid for Sfp phosphopantetheinyl transferase

    Depositor

    Christopher T Walsh, Jun Yin

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    Sfp
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pComb3

    Plasmid

    #63888

    Purpose

    For phage display of antibody libraries by fusion to modified geneIII.

    Depositor

    Carlos Barbas

    Article

    Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

    Type

    Empty backbone

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH48

    Plasmid

    #131861

    Purpose

    Produces holo-Cph1* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    Cph1(1-514,Y263F)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTrcHIS-wt7D12

    Plasmid

    #125268

    Purpose

    Expresses wild type 7D12 anti-EGFR nanobodies

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    7D12 nanobody
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TDP-43_CTD_M337V

    Plasmid

    #98674

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M337V

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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