30,410 results
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Plasmid#110097PurposeAnalogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
UseTagsbdSUMOExpressionBacterialMutationPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB107a
Plasmid#79218PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available SinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Blue
Plasmid#220782PurposeExpresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertblue chromoprotein (aeBlue)
UseTags8xHisExpressionBacterialMutationPromoterAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed trigger
Plasmid#107357PurposeTrigger sequence for pNP1 eforRed toehold switchDepositorInserttoehold trigger 1
UseTagsExpressionBacterialMutationPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB064d
Plasmid#79197PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlac62; Ppro1Available SinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAT13araE
Plasmid#18987DepositorAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGMC00018 (aka pRC0082)
Plasmid#195320PurposeMinimal plasmid backbone with multiple cloning site for HDR donor DNA constructionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only