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32,115 results

Showing: 1921 - 1950 of 32115 results

  1. pRibo-Tt

    Plasmid
    #154124
    Purpose
    Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells
    Depositor
    Alexander Mankin
    Article
    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:
    Inserts
    engineered bacterial rRNAs operon under control of pL promotor
    bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Ptac and pL
    Available Since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154124-plasmid-map-sequence-id-308059

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  3. p1A

    Plasmid
    #162692
    Purpose
    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162692-plasmid-map-sequence-id-324151

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  4. pET-FleBt

    Plasmid
    #149302
    Purpose
    "Enhances the yields of soluble target proteins in E. coli expression system", "Enable quick and efficient protein purification with high purity and stability"
    Depositor
    Michael Kolbe
    Article
    Wang et al PLoS Pathog. 2021 Nov 17;17(11):e1010052
    Type
    Empty backbone
    Tags
    FleBt and HRV 3C protease recognition site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    149302-plasmid-map-sequence-id-311624

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  6. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

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  9. pGLO-Yellow-AZA

    Plasmid
    #220783
    Purpose
    Expresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    yellow chromoprotein
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    220783-plasmid-map-sequence-id-444833

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  12. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

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  19. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  21. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available Since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  23. p406TEF1

    Plasmid
    #15976
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

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  25. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106904-plasmid-map-sequence-id-206325

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Showing: 1921 - 1950 of 32115 results