29,981 results

1. pT7-7 asyn WT

   Plasmid

   #36046

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115207

   Purpose

   Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   BzoCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Use

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIC28-BSA4-G-L11

   Plasmid

   #127830

   Purpose

   Production of recombinant human ribosomal protein L11

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPL11 (RPL11 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCrepusculo

   Plasmid

   #190156

   Purpose

   Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

   Depositor

   Andreas Moeglich

   Article

   Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

   Type

   Empty backbone

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2CT-His-MBP-Lbu_C2c2_WT

   Plasmid

   #83482

   Purpose

   Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWUR 1+2 tetO mut89

   Plasmid

   #80102

   Purpose

   Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1 (cas1 )
   Cas2 (cas2 )
   Cas1 (cas1 )
   Cas2 (cas2 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q24H, P202Q, G241D, E276D, L297Q

   Promoter

   Available since

   Dec. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEvol-pAzFRS.1.t1

   Plasmid

   #73547

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.1.t1
   pAzFRS.1.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115220

    Purpose

    Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin3Cas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge