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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pProEx-IDE-wt

Plasmid

#99014

Purpose

Express human Insulin Degrading Enzyme (IDE) in E. coli.

Depositor

Wei-Jen Tang

Publication

Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

Insert

insulin degrading enzyme (IDE Human)

Tags

His tag

Expression

Bacterial

Promoter

TRC

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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KillerOrange /pQE-30

Plasmid

#74748

Purpose

KillerOrange gene for bacterial expression

Depositor

Konstantin Lukyanov

Publication

Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

Insert

KillerOrange

Tags

HisTag

Expression

Bacterial

Mutation

KillerRed with substitutions G3C Y66W D113S N145S…

Promoter

T5

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Publication

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET32bplus_trx_dAChE4

Plasmid

#83917

Purpose

Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

Depositor

Sarel Fleishman

Publication

Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

Insert

Designed hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)

Tags

His, S-tag, and TRX

Expression

Bacterial

Mutation

V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…

Promoter

T7 promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pZA16mflon

Plasmid

#75439

Purpose

Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Publication

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pMAL-c4X

Plasmid

#75288

Purpose

Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

Depositor

Paul Riggs

Publication

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available since

July 19, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pComb3XLambda

Plasmid

#63892

Purpose

Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

human antibody lambda light chain (IGLL5 Human)

Use

Phage display

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCWori cytochrome P450-BM3h 9D7

Plasmid

#61308

Purpose

Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

Depositor

Alan Jasanoff

Publication

Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

Insert

Cytochrome P450-BM3 heme domain 9D7

Tags

6xHis

Expression

Bacterial

Mutation

T268A, I263A, T438V, A328G

Promoter

Tac

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Publication

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and TurboRFP

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
mRuby2-pBAD

Plasmid

#54771

Purpose

Localization: Protein Expression Vector , Excitation: 559, Emission: 600

Depositor

Michael Davidson

Publication

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and mRuby2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pCIOX

Plasmid

#51300

Purpose

E.coli expression vector for His8-SUMO tagged recombinant protein

Depositor

Andrea Mattevi

Type

Empty backbone

Tags

His8 + SUMO

Expression

Bacterial

Promoter

T7

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRSETa_ mEos4a

Plasmid

#51072

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant

Depositor

Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4a

Expression

Bacterial

Promoter

T7

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSt-KO

Plasmid

#44566

Purpose

for generation of knockout of mycobacterial genes

Depositor

Vinay Nandicoori

Publication

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Expression

Bacterial

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pST-2K

Plasmid

#44564

Purpose

for ectopic constitutive expression of two mycobacterial proteins in one vector

Depositor

Vinay Nandicoori

Publication

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Publication

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Publication

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Tags

GST

Expression

Bacterial

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pACYC-T7

Plasmid

#41187

Depositor

Dan Bolon

Publication

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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