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pMV306G13+Lux

Plasmid

#26160

Depositor

Siouxsie Wiles, Brian Robertson

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Bacterial luciferase operon

Use

Tags

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pM1s3TsR

Plasmid

#137922

Purpose

E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology; For use with e. coli nissle in…

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Use

Tags

GST Tag

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Use

Tags

GFP mut3

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

Plasmid

#176742

Purpose

Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS5c-mCherry-AsLOV2*(543)

Plasmid

#202712

Purpose

Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Insert

mCherry-AsLOV2*(543)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTHSSe_49

Plasmid

#109247

Purpose

pSC101 var7 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Use

Tags

Expression

Bacterial

Mutation

abCAS(D537A)

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pB-Rex

Plasmid

#122134

Purpose

B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

B. subtilis Rex

Use

Tags

Expression

Bacterial

Mutation

Promoter

apFAB338

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pDuet TDP43 WT

Plasmid

#27462

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Use

Tags

Expression

Bacterial

Mutation

R632S

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO PPM1H

Plasmid

#207333

Purpose

Bacterial expression of His SUMO PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-E01DARPin

Plasmid

#198287

Purpose

Produces PVCs retargeted with anti-EGFR DARPin E01

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-E01DARPin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLN-AIDA-Tyr1

Plasmid

#192831

Purpose

IPTG inducible AIDA-Tyrosinase expression

Depositor

André Studart

Article

Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

Insert

AIDA-Tyr1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pL-lac

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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