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pOPINK-OTUD1 (full-length, aa 1-481)

Plasmid

#61405

Purpose

Expresses human OTUD1 (full-length) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pS3

Plasmid

#50596

Purpose

Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

Depositor

Jay Keasling

Publication

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Inserts

aroE
aroD
aroB
aroG*
ppsA
tktA

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mT-Sapphire-pBAD

Plasmid

#54571

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Type

Empty backbone

Tags

6xHis and mT-Sapphire

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pST-KiT

Plasmid

#44562

Purpose

for integrative inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Publication

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTD-CTwinStrep

Plasmid

#45939

Purpose

use to create C-terminal Twin-Strep-tag fusion proteins

Depositor

Thorben Dammeyer

Publication

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pPROEX Aqua

Plasmid

#42889

Depositor

Fabienne Merola

Publication

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

T65S and H148G

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-21a(+)/MBP-His

Plasmid

#38006

Depositor

Michael J Fox Foundation MJFF

Insert

MBP (Maltose binding protein)

Tags

6xHIS

Expression

Bacterial

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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human p53-(82-393)

Plasmid

#24867

Depositor

Cheryl Arrowsmith

Publication

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 82-393

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBS-hsFlt3L

Plasmid

#25074

Depositor

Maria Castro

Publication

Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

Insert

human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)

Expression

Bacterial

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-145

Plasmid

#22291

Depositor

Pietro De Camilli

Publication

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Col1a-3'probe

Plasmid

#20731

Depositor

Rudolf Jaenisch

Publication

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1a-3'probe (Col1a1 Mouse)

Expression

Bacterial

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pE3n

Plasmid

#17457

Purpose

modified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Publication

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

6xMyc

Expression

Bacterial, Insect, Mammalia…

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pE3c

Plasmid

#17463

Purpose

modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Publication

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

6xMyc

Expression

Bacterial, Insect, Mammalia…

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2C

Plasmid

#15779

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2C (UBE2C Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2M

Plasmid

#15798

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2M (UBE2M Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Nkx2.1

Plasmid

#15540

Depositor

John Rubenstein

Publication

Shimamura et al Development. 1995 Dec . 121(12):3923-33.

Insert

Nkx2.1 (Nkx2-1 Mouse)

Expression

Bacterial

Available since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only

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6972 pPROEX-HTb-SSA1

Plasmid

#1231

Depositor

Susan Lindquist

Publication

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ssa1 (SSA1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-mNeongreen

Plasmid

#182387

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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