34,359 results
-
-
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorInsertsMutL E32K
Lambda Beta
XylS
ExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-N-HA
Plasmid#119756PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.DepositorTypeEmpty backboneTagsGST and HAExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
ExpressionBacterialPromoterpLacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneTagsHis8-SumoExpressionBacterialPromoterT7Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMyC-kan
Plasmid#84692PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAcetamidaseAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD
Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAL-p2X
Plasmid#75287PurposeExpresses proteins in the periplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable sinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pTKEI-Dest
Plasmid#79784PurposeExpression plasmid - BsmBI entry sitesDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterpTrcAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTiGc
Plasmid#78314PurposeMycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).DepositorInsertsTurboFP635
GFP
UseSynthetic Biology; Mycobacterial replicating vect…ExpressionBacterialPromoterhsp60 and hsp60(ribo)Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINK-OTUD1 (full-length, aa 1-481)
Plasmid#61405PurposeExpresses human OTUD1 (full-length) in E. coli.DepositorAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mT-Sapphire-pBAD
Plasmid#54571PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 511DepositorTypeEmpty backboneTags6xHis and mT-SapphireExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1-HA-MEK1
Plasmid#53159PurposeExpresses GST-HA tagged human MEK1 from bacterial expression vectorDepositorAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST-KiT
Plasmid#44562Purposefor integrative inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
