32,115 results
-
Plasmid#212837PurposeExpresses the Rhizobium grahamii LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP02
Plasmid#212835PurposeExpresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP06
Plasmid#212839PurposeExpresses the Rhizobium acidisoli LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad-CAts TAG97
Plasmid#105668Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 97PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasX1
Plasmid#87685Purposep15A plasmid expressing CasX locus from DeltaproteobacteriaDepositorInsertCasX1
ExpressionBacterialPromoterNoneAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
ExpressionBacterialPromoterPoxyR, PoxySAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTCF156 HLA-A*02:01
Plasmid#180456PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
ExpressionBacterialPromoterpj23119Available SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
TagsN-terminal His6 tagExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQFmcs-2H12.D11-scarREF
Plasmid#221184PurposecdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)DepositorInsertscdGreen2
mScarlet-I
ExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterNone (same as cdGreen2) and PQ5 (cumate-inducible)Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-152
Plasmid#118534PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-anti-AlfaTag nanobody
Plasmid#171819PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInserttdTomato-anti-AlfaTag nanobody
Tags6xHis and SEP tag (6xD)ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterrrnB P1Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a 6xHis PreScission SARS-CoV-2 nsp13
Plasmid#159390PurposeExpresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.DepositorInsertN-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
TagsHis6 tagExpressionBacterialPromoterT7Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
TagsmCherryExpressionBacterialAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HIS2-Snf7
Plasmid#21492DepositorAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA-E11)2
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLE, PrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
TagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG1K
Plasmid#71741PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMyNT
Plasmid#42191DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterColE1Available SinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-TACC3-His6
Plasmid#69106PurposeFor bacterial expression of recombinant human TACC3 tagged with GST and His.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
TagsGST and His6ExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (u-msfGFP)
Plasmid#29772DepositorTypeEmpty backboneTagsSiriusGFPExpressionBacterialPromoterT7Available SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X_MBP-CLmN-H6
Plasmid#234487PurposeMaltose binding protein N-terminally fused to His-tagged CLmN split inteinDepositorInsertN-terminal Fragment of the CLm Split Intein
TagsHexahistidine Tag and Maltose Binding Protein (MB…ExpressionBacterialMutationT69K, F75H, M118N within the N-terminal fragment …Available SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-EGFP-HaloTag2
Plasmid#41742DepositorInsertHalotag 2
ExpressionBacterial and MammalianPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-142
Plasmid#118558PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMCD70
Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneTagsLacZ'ExpressionBacterialAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only