30,738 results

1. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Tags

   6-HIS

   Expression

   Bacterial

   Promoter

   T7/lac operon

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1 ElonginB, ElonginC

   Plasmid

   #230999

   Purpose

   Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Inserts

   Elongin B (Elob Mouse)
   Elongin C (Eloc Mouse)
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAR357_SFP_pCDF-1b

   Plasmid

   #122851

   Purpose

   Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   Sfp
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDJSVT87

   Plasmid

   #109302

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-KIRIN1

   Plasmid

   #107414

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Expression

   Bacterial

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-6XHis-SUMO-SIRT2

   Plasmid

   #102622

   Purpose

   Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Tags

   6XHis-SUMO

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-37 and 357-389

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-SH3(2)-5R

   Plasmid

   #112089

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-5R (NCK1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only repeats of the second SH3…

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS-mClover3

   Plasmid

   #74236

   Purpose

   Constitutive expression of mClover3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Has Service

   Cloning Grade DNA

   Insert

   mClover3
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMal-Abl-PRM 5R

    Plasmid

    #112088

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-HA-AR-NTD

    Plasmid

    #171235

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-D2

    Plasmid

    #37470

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D2 second generation cameleon (calcium sensor)
    

    Expression

    Bacterial

    Promoter

    Ara

    Available Since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET28sumo-hRig-I-Full-length

    Plasmid

    #210827

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

    Insert

    hRIG-I (DDX58) Full length protein (RIGI Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-(pos36)GFP

    Plasmid

    #62937

    Purpose

    Expression of 6xHis-(+36)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (pos36)GFP
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMG046 pETARA-CK1α-His

    Plasmid

    #154074

    Purpose

    Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T1 SHP2 WT

    Plasmid

    #8322

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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