29,691 results

1. pAureus-TnCAM

   Plasmid

   #199807

   Purpose

   vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

   Depositor

   Steve Salipante

   Article

   Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

   Insert

   transposon cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-28a-mCherry-MED1-IDR

   Plasmid

   #194545

   Purpose

   Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   MED1 (MED1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-A11(2-196)-Strep

   Plasmid

   #196208

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-TEV-PRC1

   Plasmid

   #167539

   Purpose

   For expression of His6-TEV-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKTrfp

   Plasmid

   #59772

   Purpose

   pKT230 derivative; PBAD_rfp; cmR

   Depositor

   Nathan Hillson

   Article

   Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

   Insert

   rfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-T-rnVAPA-1-219

   Plasmid

   #13395

   Depositor

   Axel Brunger

   Article

   Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

   Insert

   VAP-A (Vapa Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pBR322-Pdp1_NTD-Cre

   Plasmid

   #198274

   Purpose

   For loading Cre into PVCs; also contains PVC regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-Cre
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCW-LIC-N8C_Fab3b

   Plasmid

   #203685

   Purpose

   Expresses N8C_Fab3b

   Depositor

   Brenda Schulman

   Article

   Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

   Insert

   N8C_Fab3b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmMaple3-CAM

    Plasmid

    #101148

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET32bplus_trx_dAChE4

    Plasmid

    #83917

    Purpose

    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

    Depositor

    Sarel Fleishman

    Article

    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

    Insert

    Designed hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    

    Use

    Tags

    His, S-tag, and TRX

    Expression

    Bacterial

    Mutation

    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…

    Promoter

    T7 promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFGL821

    Plasmid

    #58223

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    hygromycin B phosphotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDD132

    Plasmid

    #119885

    Purpose

    Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    BbaJ23104_EYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-HaloTag-KanMX6

    Plasmid

    #87029

    Purpose

    Tag S. pombe genes in C terminal with HaloTag

    Depositor

    Julien Berro

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTEF2

    Plasmid

    #43971

    Depositor

    Hal Alper

    Article

    Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF

    Available since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAB094a

    Plasmid

    #79241

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4; PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIOM15

    Plasmid

    #20869

    Depositor

    Carmen Beuzón

    Article

    Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

    Insert

    aphA::aadA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009 . 464():211-31.

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pKS17

    Plasmid

    #24628

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    pdc, adhB, atfA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge