30,040 results

1. pGDP3 vatD

   Plasmid

   #112885

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   vatD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGDP3 aph(3'')-Ia

   Plasmid

   #112879

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGDP3 aph(9)-Ia

   Plasmid

   #112882

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   aph(9)-Ia
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. prhaBAD-anti-mouse-Nb-Hia5

   Plasmid

   #239626

   Purpose

   Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

   Depositor

   Aaron Straight

   Article

   Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

   Insert

   Hia5
   

   Use

   Tags

   6HIS, MBP, TEV, and anti-Mouse Nanobody

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   rhaBAD

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. prhaBAD-anti-rabbit-Nb-Hia5

   Plasmid

   #239627

   Purpose

   Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

   Depositor

   Aaron Straight

   Article

   Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

   Insert

   Hia5
   

   Use

   Tags

   6HIS, MBP, TEV, and anti-Rabbit Nanobody

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   rhaBAD

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG GST Empty

   Plasmid

   #236735

   Purpose

   Empty bacterial expression plasmid for GST fusion proteins

   Depositor

   Melina Fan, Addgene Research Program

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAG GST EGFP

   Plasmid

   #236736

   Purpose

   Bacterial expression plasmid containing GST-tagged EGFP

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   EGFP
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   April 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKEK3395

   Plasmid

   #234079

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   YukonOFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-22b(+) pCopA RBS30 mscarlet

    Plasmid

    #226370

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    mScarlet
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA)

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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