30,535 results
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Plasmid#178467PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertAP2B1_adaptin (AP2B1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNDGG054
Plasmid#231323PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneUseTagsmyc, HisExpressionBacterialMutationPromoterAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
UseTags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AckRS
Plasmid#137976Purposesequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansionDepositorInsertAckRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBWB424
Plasmid#140638PurposeRSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-msGFP2
Plasmid#135301PurposeEncodes a photostable monomeric superfolder GFPDepositorInsertmsGFP2
UseTagsExpressionBacterialMutationPromoterT5Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCHERRY3
Plasmid#24659DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable SinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRBV7-9
Plasmid#123437Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV26-1
Plasmid#123398Purposeconstruction of TCRDepositorAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and mGFPExpressionBacterial and InsectMutationPromoterAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTNS2
Plasmid#87802PurposeFor constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-E1103G
Plasmid#10989DepositorInsertrpb1-E1103G (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 3308 A to G in the RPB1 ORF resulting in…PromoterAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAC3-tag-N
Plasmid#185482PurposeGateway compatible destination vectors containing StrepIII/HA/UltralDDepositorTypeEmpty backboneUseTagsStrepIII-HA-UltraIDExpressionBacterial and MammalianMutationPromoterAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only