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pRSET-B mCherry

Plasmid

#108857

Purpose

Encodes for the fluorescent protein mCherry

Depositor

Kalina Hristova

Publication

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

mCherry

Expression

Bacterial

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pIB184 (EM)

Plasmid

#90194

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Publication

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD miniSOG

Plasmid

#91774

Purpose

Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

Depositor

Erik Rodriguez, Roger Tsien

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

mini Singlet Oxygen Generator

Tags

Hexa-Histidine tag

Expression

Bacterial

Promoter

araBAD

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-MBP-Geo_st

Plasmid

#87700

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

Depositor

Jennifer Doudna

Insert

GeoCas9

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Publication

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Publication

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p2CT-His-MBP-Lsh_C2c2_WT

Plasmid

#83487

Purpose

Bacterial expression of WT L. shahii C2c2 CRISPR effector

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lsh_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBAD_Kan-LacI

Plasmid

#79826

Purpose

Arabinose induced expression of Lac repressor

Depositor

Katja Arndt

Publication

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Insert

LacI

Tags

His6

Expression

Bacterial

Promoter

araBp

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Publication

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-4T1-RhoA G17A

Plasmid

#69357

Purpose

expression of nucleotide free RhoA in bacterial cells

Depositor

Rafael Garcia-Mata

Publication

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Mutation

G17A

Promoter

tac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pHYRSF53

Plasmid

#64696

Purpose

His-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Publication

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, and NpuDnaE-N102

Expression

Bacterial

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Publication

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PpsbA2

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAWP89

Plasmid

#61264

Purpose

IncP-based replicating vector expressing dTomato under tac promoter.

Depositor

Mary Lidstrom

Publication

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Insert

Ptac-dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

tac

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YPet-pBAD

Plasmid

#54860

Purpose

Localization: Protein Expression Vector , Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Publication

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

6xHis and YPet

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pACλCI

Plasmid

#53730

Purpose

PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

Depositor

Ann Hochschild

Publication

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

bacteriophage lambda CI protein

Expression

Bacterial

Promoter

lac UV5

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV306DIG13+FFlucRT

Plasmid

#49998

Purpose

Integrase removed to increase stability of the plasmid in mycobacteria

Depositor

Brian Robertson

Publication

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Publication

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTrcHisB-TRF2

Plasmid

#50488

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Publication

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-500

Promoter

pTrc

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pOSIP-CT (CamR, P21)

Plasmid

#45981

Depositor

Drew Endy, Keith Shearwin

Publication

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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