30,738 results

1. pSm-Nuclease

   Plasmid

   #132431

   Purpose

   Expresses Serratia marcescens nuclease in E. coli using T7 promoter

   Depositor

   Mark Arbing

   Insert

   Serratia marcescens nuclease (codon optimized)
   

   Tags

   None

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LVXN-Neo-NSD2

   Plasmid

   #86010

   Purpose

   express wild-type NSD2 in mammalian cells

   Depositor

   Darrin Stuart

   Article

   Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

   Insert

   NSD2 (NSD2 Human)
   

   Use

   Lentiviral

   Tags

   Flag tag D Y K D D D D K

   Expression

   Bacterial

   Mutation

   K1002R (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCYP64

   Plasmid

   #212897

   Purpose

   Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Expression

   Bacterial

   Mutation

   P450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…

   Promoter

   PcymRC

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SepOTSλ

   Plasmid

   #68292

   Purpose

   Phosphoserine orthogonal translation system (preferred variant)

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EFSep21, 4xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. M13KO7-ΔPS-ΔgeneIII

   Plasmid

   #134351

   Purpose

   Helper phage for M13 phage production

   Depositor

   Mark Isalan

   Article

   Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

   Insert

   Helper phage gene
   

   Expression

   Bacterial

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR83

   Plasmid

   #90211

   Purpose

   Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

   Inserts

   OmpR (ompR E. coli)
   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCBP1_Full-length_pGEX

   Plasmid

   #135108

   Purpose

   Recombinant expression of PCBP1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP1 (PCBP1 Human)
   

   Tags

   GST-SBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBEI-6409

   Plasmid

   #47048

   Purpose

   BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Expression

   Bacterial

   Promoter

   PlacUV5

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11a-Z-NspGFP

   Plasmid

   #40729

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   N super positive GFP - Leucine Zipper
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDule-Tet2.0

    Plasmid

    #85496

    Purpose

    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

    Insert

    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32G L65Q F108S Q109D D158S L162N

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-MBP-BrCas12b-RFND

    Plasmid

    #195339

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis and 6xHis, MBP, TEV cleavage site

    Expression

    Bacterial

    Mutation

    R160E, F208W, N524V, D868V

    Promoter

    T7

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWPSG-Po-nCas9-BE

    Plasmid

    #195740

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Po (phoenol-inducible promoter)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218857

    Purpose

    Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Tags

    QF fusion protein

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. F-IRF7

    Plasmid

    #169777

    Purpose

    Express IRF7 in mammalian cells

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)
    

    Tags

    Flag tag

    Expression

    Bacterial and Mammalian

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3-ARF6-His6(CO)

    Plasmid

    #201887

    Purpose

    Used for bacterial expression of human protein ARF6

    Depositor

    Richard Kahn

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbS5a-Opto-Cre-Vvd-2

    Plasmid

    #160400

    Purpose

    Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd-2
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSerOTS-C1* (V70)

    Plasmid

    #188537

    Purpose

    Phosphoserine incorporation into recombinant proteins

    Depositor

    Jesse Rinehart

    Article

    Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

    Inserts

    pSerRS
    Ef-pSer
    tRNApSer
    

    Expression

    Bacterial

    Mutation

    mutations resulting from laboratory evolution

    Promoter

    glnS*

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE-Tn5-165

    Plasmid

    #118545

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMT3_SRSF1

    Plasmid

    #201056

    Purpose

    Express sumo-tagged human SRSF1

    Depositor

    Jun Zhang

    Article

    Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

    Insert

    SRSF1 (SRSF1 Human)
    

    Tags

    sumo

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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