30,260 results

1. pPM2213

   Plasmid

   #167553

   Purpose

   Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

   Depositor

   Renato Morona

   Article

   Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

   Insert

   rfb region of Shigella flexneri serotype 2a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAD-sfGFP(Sp)-Pman

   Plasmid

   #133833

   Purpose

   Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(Sp)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK1010 (pSL25)

   Plasmid

   #191005

   Purpose

   Expresses blue chromoprotein on a broad host range RP4 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   blue chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT85_P438-mEOS3.2_GentaR

   Plasmid

   #173894

   Purpose

   Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

   Depositor

   Yonathan Arfi

   Article

   Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

   Insert

   mEos3.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P438

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSlb-wt

   Plasmid

   #60728

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINVHH_BAP_his

   Plasmid

   #210402

   Purpose

   For expression of VHH with C-terminal biotin acceptor peptide and his tag

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Use

   Tags

   biotin acceptor peptide-his6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET264-pUC 24xMS2V6 Loxp KANr Loxp

   Plasmid

   #104393

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPomyces-SaCas9

   Plasmid

   #129553

   Purpose

   Contains codon-optimized SaCas9 and gRNA for streptomyces

   Depositor

   Fong Tian Wong, Mingzi M. Zhang

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   SaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pShHELIX_sgRNA_entry (CJT32)

   Plasmid

   #181782

   Purpose

   Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pORTMAGE-3

    Plasmid

    #72678

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3CEG

    Plasmid

    #60107

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BIRC6:SDC136-F10:C41228 (BIRC6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pBPHLWL-LexA

    Plasmid

    #26258

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    LexA
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis-BRD4 BD1

    Plasmid

    #196544

    Purpose

    Expression of BRD4 Bromodomain (BD1) in E.coli

    Depositor

    Byung Il Lee

    Article

    Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

    Insert

    BRD4 BD1 (BRD4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCM001

    Plasmid

    #124605

    Purpose

    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

    Depositor

    David Summers

    Article

    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

    Insert

    mCherry-pHluorin
    

    Use

    Tags

    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

    Expression

    Bacterial

    Mutation

    Promoter

    pDAB

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEL1200

    Plasmid

    #138526

    Purpose

    For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

    Insert

    mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)
    

    Use

    Tags

    N-terminal His tag and TEV cleavage site on MlaD

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-HTNC

    Plasmid

    #13763

    Purpose

    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

    Depositor

    Klaus Rajewsky

    Article

    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

    Insert

    His-TAT-NLS-Cre
    

    Use

    Baculovirus

    Tags

    His Tag, NLS, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    p10 promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSW036

    Plasmid

    #140034

    Purpose

    KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cpt

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-mCherry-PA-Rac1

    Plasmid

    #22027

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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