33,434 results
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Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-SUMO-mGSDMD
Plasmid#111560PurposeExpress mouse GSDMD in E.coliDepositorAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET-B mCherry
Plasmid#108857PurposeEncodes for the fluorescent protein mCherryDepositorInsertmCherry
ExpressionBacterialAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorPublicationInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-SCFP3A
Plasmid#103970PurposePlasmid encoding SCFP3ADepositorInsertSCFP3A
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Keap1
Plasmid#62454PurposeHis6 tagged Keap1 for E.coli expressionDepositorPublicationAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSCB-YFP
Plasmid#101737PurposeThe plasmid can be either plasmid propogation backbone or expression vector in specific strainDepositorTypeEmpty backboneUseSynthetic BiologyTagseYFPExpressionBacterialPromoterTrc1OAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC018 - LshCas13a from Leptotrichia shahii
Plasmid#91908PurposeExpresses LshCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLshCas13a and guide expression cassette
ExpressionBacterialAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SpyCatcher-ELP-GFP
Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
Tags6x His Tag, GFP, and TEV TagExpressionBacterialPromoterT5 promoter/lac operator elementAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKD236-4b
Plasmid#90956PurposeExpresses ThsS constitutivelyDepositorInsertThsS
ExpressionBacterialPromoterBba_J23104Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2CT-His-MBP-Lse_C2c2_WT
Plasmid#83486PurposeBacterial expression of WT L. seeligeri C2c2 CRISPR effectorDepositorInsertLse_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pO_72
Plasmid#78235PurposeTranscripts the r_oligo part that includes the HIV reverse transcriptase binding site (HTBS)DepositorInsertr_oligo
ExpressionBacterialPromoterj23100Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
ExpressionBacterialAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags(-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas1+2
Plasmid#72676PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.DepositorInsertCas1, Cas2
ExpressionBacterialAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZ003 (LshC2c2 locus)
Plasmid#70168PurposeExpresses LshC2c2 full locusDepositorInsertLshC2c2 full locus
ExpressionBacterialAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMCSG17-NleL
Plasmid#66716PurposeExpresses NleL (170-782) in E. coliDepositorAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -