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Plasmid#153313PurposeBacterial expression of the Bst Large FragmentDepositorInsertBst Large Fragment
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationSee manuscriptPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPFO*
Plasmid#120400PurposeExpresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.DepositorInsertMutant form of Perfringolysin O
UseTagsHis6 tagExpressionBacterialMutationAmino acids 1-27 are deleted, starts at amino aci…PromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN6
Plasmid#203684PurposeExpresses red fluorescent marker DsRed-Express in Listeria monocytogenesDepositorInsertDsRed-Express
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5RGDPK7
Plasmid#198291PurposeProduces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)DepositorInsertPVCpnf_pvc13-Ad5RGDPK7
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xHA
Plasmid#129567PurposeEmpty backbone for HA tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET24b-SUMO-BG2.4
Plasmid#199438Purposeexpresses BAmGreen2.4 in bacterial cells with a SUMO tagDepositorInsertBAMGreen2.4
UseTagshis tag and sumoExpressionBacterialMutationPromoterT7Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
luxB:cp157Venus+luxA/pRSETB Duet
Plasmid#197583PurposeBRET lux luciferase with cp157Venus for bacterial expressionDepositorInsertLuxA LuxBcp157Venus
UseE.coli expressionTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
UseTagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-6xHis mAb
Plasmid#214952PurposeBacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain
UseTagsVNp6ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216
Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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LVXN-Neo-NSD2
Plasmid#86010Purposeexpress wild-type NSD2 in mammalian cellsDepositorInsertNSD2 (NSD2 Human)
UseLentiviralTagsFlag tag D Y K D D D D KExpressionBacterialMutationK1002R (please see depositor comments below)PromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_42
Plasmid#109240PurposepSC101 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn-pOUT
Plasmid#213905PurposeMariner transposon with outward-facing pJ23104 promoterDepositorInsertmariner transposon with kan resistance and outward-facine pJ23104 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only