30,416 results

1. pULTRA-CNF

   Plasmid

   #48215

   Purpose

   highly efficient suppressor plasmid

   Depositor

   Peter Schultz

   Article

   Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

   Inserts

   polyspecific MJ tyrosyl synthetase
   tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32L, L65V, F108W, Q109M, D158G, I159A

   Promoter

   proK and tacI

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AP1

   Plasmid

   #179678

   Purpose

   Accessory plasmids

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNeg-Ma-barnase-TAG3-TAG45

   Plasmid

   #197572

   Purpose

   Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Barnase - 2xTAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   3TAG and 45TAG

   Promoter

   araC and lpp

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPO2

   Plasmid

   #209976

   Purpose

   Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (SH71_rep)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. plRL117

   Plasmid

   #163635

   Purpose

   Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRBBm34

   Plasmid

   #48114

   Purpose

   Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138128

   Purpose

   Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYZ205

    Plasmid

    #104586

    Purpose

    Express SNAP-6His-CBD

    Depositor

    New England Biolabs, Yinhua Zhang

    Insert

    SNAP-6His-CBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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