5,046 results
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Plasmid#147580PurposeInsect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666DepositorInsertDm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)
ExpressionInsectAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-FlucV5His6-PolyA-HhR_AB
Plasmid#148303PurposeInsect Expression of PolyA-HhRDepositorInsertPolyA-HhR
ExpressionInsectAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q
Plasmid#147340PurposeInsect Expression of DmNot4-dsRNAresDepositorInsertDmNot4-dsRNAres (CG31716 Fly)
ExpressionInsectMutation7 silent mutations and 6 non silent mutations G20…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-L36AL39A_T
Plasmid#147604PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
ExpressionInsectMutationTwo non silent mutations (H628R and N659S) compar…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q
Plasmid#147279PurposeInsect Expression of DmNot3-dsRNAresDepositorInsertDmNot3-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q
Plasmid#147274PurposeInsect Expression of DmeIF4Ga-Y80A-dsRNAresDepositorInsertDmeIF4Ga-Y80A-dsRNAres (eIF4G1 Fly)
ExpressionInsectMutationone non silent mutation K340R compared to the seq…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q
Plasmid#147262PurposeInsect Expression of DmNot3-F818A-dsRNAresDepositorInsertDmNot3-F818A-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-10xmut_G
Plasmid#146428PurposeInsect Expression of DmGW182-10xmutDepositorInsertDmGW182-10xmut (gw Fly)
ExpressionInsectMutationMultiple mutations compared to NM_166780.1, see a…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P
Plasmid#147209PurposeInsect Expression of DmXRN1-P1339AP1342A-dsRNAresDepositorInsertDmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J
Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
ExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
ExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R
Plasmid#147394PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389ADepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Human, Nematode)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O
Plasmid#147065PurposeInsect Expression of DmXRN1-D207A-dsRNAresDepositorInsertDmXRN1-D207A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N
Plasmid#146969PurposeInsect Expression of DmXRN1-del1323-1355-dsRNAresDepositorInsertDmXRN1-del1323-1355-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delCterm_E
Plasmid#146252PurposeInsect Expression of DmGW182-delC-termDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E
Plasmid#146241PurposeInsect Expression of DmSMG6-siRNAresDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
TagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔN
Plasmid#182840PurposeA 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
TagsFLAG tagExpressionInsectPromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
TagsmCherryExpressionInsectPromoternanosAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::FRT,attB}
Plasmid#182180Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertmTurquoise
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-EGFP::GW,attB}
Plasmid#182170Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertEGFP
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::GW,attB}
Plasmid#182169Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertmTurquoise
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3DsR
Plasmid#182204Purpose1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-DsRed
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G
Plasmid#146359PurposeInsect Expression of DmDCP2-PBDepositorInsertDmDCP2-PB (DCP2 Fly)
ExpressionInsectMutationContains an in-frame insertion that adds a Q afte…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2_E
Plasmid#146224PurposeInsect Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
ExpressionInsectMutationContains a K660E mutation compared to the sequenc…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F
Plasmid#146286PurposeInsect Expression of HsAGO2-F2V2DepositorInsertHsAGO2-F2V2 (AGO2 Human)
ExpressionInsectMutationContains F470V, F505V, K660E mutations compared t…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only