5,046 results
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Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only
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mWasabi-ADAP1
Plasmid#163906PurposeFull length mouse ADAP1 with N terminal fluorescent tag for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-639 (attR4-attR2)
Plasmid#161880PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-624 (attR4-attR2)
Plasmid#161879PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
58 pLIB_SNAP
Plasmid#160307PurposeN-term 3C cleavable SNAP tag for insect cell expressionDepositorTypeEmpty backboneTags12xHis and SNAPExpressionInsectPromoterpolyhydrinAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlgb
Plasmid#140293PurposeExpress soluble His-tagged zebrafish Kitlgb in insect cellsDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlga
Plasmid#140292PurposeExpress soluble His-tagged zebrafish Kitlga in insect cellsDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
48 pLIB_C_GST
Plasmid#160273PurposeC-term uncleavable GST tag for insect cell expressionDepositorTypeEmpty backboneTagsGSTExpressionInsectPromoterpolyhydrinAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
49 pLIB_3C_C_GST
Plasmid#160274PurposeC-term 3C cleavable GST tag for insect cell expressionDepositorTypeEmpty backboneTagsGSTExpressionInsectPromoterpolyhydrinAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
908I
Plasmid#160241PurposeVanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attBDepositorInsertsVan Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
907F1
Plasmid#160233PurposeFlightN-VanR-VP16-3X-SV40-attBDepositorInsertsVanR
Flightin promoter
TagsVP16ExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045B
Plasmid#125004PurposeExpresses gRNAs targeting hid, eve, and hedgehogDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045D
Plasmid#125006PurposeExpresses gRNAs targeting hedgehog and winglessDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045E
Plasmid#125007PurposeExpresses tRNA-flanked gRNAs targeting hid, eve, and hedgehogDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986C
Plasmid#125000PurposeExpresses dCas9-VPR driven by the bottleneck promoterDepositorInsertdCas9-VPR
UsePiggybackExpressionInsectPromoterbottleneckAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986D
Plasmid#125001PurposeExpresses dCas9-VP64 driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VP64
UsePiggybackExpressionInsectPromoterubiquitinAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986E
Plasmid#125002PurposeExpresses dCas9-VP64 driven by the bottleneck promoterDepositorInsertdCas9-VP64
UsePiggybackExpressionInsectPromoterbottleneckAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1045A
Plasmid#125003PurposeExpresses gRNAs targeting hid and eveDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(1)-CRY2-mCherry
Plasmid#160440PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.DepositorAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(0)-CRY2-mCherry
Plasmid#160439PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.DepositorAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
R979-X57-008: Hs.KRAS4b(1-169)
Plasmid#159690PurposeBaculovirus protein expression of KRAS4b (1-169)DepositorInsertHs.KRAS4b(1-169) (KRAS Human)
ExpressionInsectAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
Plasmid#159557PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationG13DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
Plasmid#159559PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationQ61RAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159555PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationG12DAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
TagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050I (notch)
Plasmid#132421Purposeexpress arrays of gRNA targeting Notch under dU6-3 promoterDepositorInsertnotch gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050K (firefly luciferase)
Plasmid#132422Purposeexpress arrays of gRNA targeting Firefly Luciferase under dU6-3 promoterDepositorInsertfirefly Luciferase gRNA array
ExpressionInsectPromoterU6-3Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only