4,908 results

1. pCFD3-dU6:3gRNA

   Plasmid

   #49410

   Purpose

   expression of gRNA under control of the Drosophila U6:3 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx4-H6-FGAm (FlincG3)

   Plasmid

   #49202

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   FlincG3
   

   Use

   Tags

   His tag + other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Met to Lys in cpEGFP region similar to CGaMP3

   Promoter

   CMV

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRExpress-Bxb1-hsp70pA

   Plasmid

   #158391

   Purpose

   heat shock inducible Bxb1 integrase for drosophila melanogaster

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   Bxb1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. UAS-Ceru-10unit

   Plasmid

   #158389

   Purpose

   10-unit intMEMOIR recording array for Drosophila melanogaster

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   10unit recording array
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1-6His-DHX9

   Plasmid

   #211440

   Purpose

   generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

   Depositor

   Frank Grosse, Helmut Pospiech

   Article

   Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

   Insert

   DHX9 (DHX9 Human)
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIZ-Flag6His-Siwi

   Plasmid

   #50560

   Purpose

   Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

   Depositor

   Yukihide Tomari

   Article

   Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

   Insert

   Siwi (Aubergine )
   

   Use

   Tags

   6xHIS and FLAG

   Expression

   Insect

   Mutation

   Promoter

   OpIE2

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUASTattB-GFP-Msp300KASH

   Plasmid

   #170806

   Purpose

   Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

   Depositor

   Vikki Weake

   Article

   Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

   Insert

   GFP-Msp300KASH
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   5xUAS + hsp70 minimal promoter

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 13XLexAop2 IVS ChrimsonR mCherry_tr

   Plasmid

   #111548

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPL

    Plasmid

    #110743

    Purpose

    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCDM

    Plasmid

    #110742

    Purpose

    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. IR25a-T2A-QF-AD

    Plasmid

    #162526

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ORCO-T2A-QF-DBD

    Plasmid

    #162525

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-IMD-V5-His

    Plasmid

    #63748

    Purpose

    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    immune deficiency (imd Fly)
    

    Use

    Tags

    V5-6XHis

    Expression

    Insect

    Mutation

    *see comment below

    Promoter

    pMT

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1-Kozak-rhTRIM5alpha-PFOS

    Plasmid

    #79038

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesus TRIM5alha-PFOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Activin A (S2-2)

    Plasmid

    #184621

    Purpose

    for transfection into S2 cells and expression of Activin A protein

    Depositor

    Timothy Springer

    Article

    Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

    Insert

    INHBA (INHBA Human)
    

    Use

    Tags

    8x His - streptavidin binding peptide

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBiex-1 GFPNanobody_SNAP

    Plasmid

    #82711

    Purpose

    Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    GFP Nanobody
    

    Use

    Tags

    6xHis Purification tag, FLAG purification tag, an…

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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