4,908 results

1. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1c

   Plasmid

   #80613

   Purpose

   pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBacHTa_HCK

   Plasmid

   #224245

   Purpose

   Expression of human HCK Kinase in insect cells (SF9 cells)

   Depositor

   Andrew Mesecar

   Insert

   hck (HCK Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUASp YFP Rabx6 WT

   Plasmid

   #53479

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rabx6 (RabX6 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pFasBac+GFP-CAMSAP3

   Plasmid

   #59038

   Purpose

   for baculovirus expression of mouse CAMSAP3 in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 (Camsap3 Mouse)
   

   Use

   Tags

   His and eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1d

   Plasmid

   #80614

   Purpose

   pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOPINVH

   Plasmid

   #26041

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ-KHHHHHH
   

   Use

   Tags

   KHHHHHH and u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

   Plasmid

   #55217

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCGMbX

   Plasmid

   #42300

   Depositor

   Steven Almo

   Type

   Empty backbone

   Use

   Tags

   BiP signal sequence, His6, and V5-epitope

   Expression

   Insect

   Mutation

   Promoter

   metallothonein

   Available since

   April 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJFRC3-1XUAS-IVS-mCD8::GFP

    Plasmid

    #26216

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBIG1e

    Plasmid

    #80615

    Purpose

    pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUb RxCas13d + RxCas13d guide RNA

    Plasmid

    #176303

    Purpose

    Expresses RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid

    #214140

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 10XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111547

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB1.HMBP.PrS.AEBP2

    Plasmid

    #125165

    Purpose

    Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Zinc finger protein AEBP2 (AEBP2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

    Plasmid

    #48297

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastbac-Dual-StrepII-BARD1

    Plasmid

    #137166

    Purpose

    Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    BARD1 (BARD1 Human)
    

    Use

    Tags

    StrepII

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    p10

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiex-1 Myosin VI HMM_eGFP

    Plasmid

    #82714

    Purpose

    Expresses Myosin VI HMM with a C-terminal eGFP.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Myosin VI
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-Ls-AChBP

    Plasmid

    #63126

    Purpose

    Expression of Lymnaea stagnalis AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASTattB_NSlmb-vhhGFP4

    Plasmid

    #35577

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge