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pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Article

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Synthetic, Human)

Use

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mPA-GFP-PA-Rac1

Plasmid

#22022

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Tags

6X His and mPA-GFP

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_WT

Plasmid

#162579

Purpose

Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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AE-Polyubiquitin-GCaMP6s

Plasmid

#106868

Purpose

Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

Depositor

Omar Akbari

Article

Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

Insert

AE-Polyubiquitin-GCAaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

Ae. aegypti polyubiquitin (PUb)

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E2 OpIE2-GFP

Plasmid

#173505

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
EGFP

Use

Tags

Expression

Insect

Mutation

Nix isoforms with exons 1, 2 separated by intron 1

Promoter

OpIE2 and own

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Use

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

Plasmid

#214139

Purpose

Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB1 (PSMB1 Human)
PSMB2 (PSMB2 Human)
PSMB3 (PSMB3 Human)
PSMB4 (PSMB4 Human)
PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Use

Tags

2xSTII

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_ybbR_Tau

Plasmid

#159717

Purpose

Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

Microtubule-associated protein tau (MAPT Human)

Use

Tags

ZZ affinity tag, ybbR tag

Expression

Insect

Mutation

Promoter

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Use

Tags

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_053

Plasmid

#216083

Purpose

for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31

Use

Destination

Tags

Expression

Insect

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCasper_Pdf-LexA::VP16

Plasmid

#33259

Depositor

Michael Rosbash

Article

Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

Insert

LexA::VP16-SV40 3'UTR (lexA E. coli)

Use

Tags

Expression

Insect

Mutation

Promoter

Pdf

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pUASTattB_NSlmb-vhhGFP4

Plasmid

#35577

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag BAF155 b

Plasmid

#1954

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BAF155 (SMARCC1 Human)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac1_GI.1_N116C-G193C

Plasmid

#162580

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Asparagine 116 was mutated to Cysteine and Glycin…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
R979-X58-008: Hs.KRAS4b(1-188)

Plasmid

#159691

Purpose

Baculovirus protein expression of KRAS4b (1-188)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-188) (KRAS Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFBD-ESCRT 0

Plasmid

#21499

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)

Use

Tags

GST and His-MBP

Expression

Insect

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only