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Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC150-20XUAS-IVS-Flp1::PEST
Plasmid#32132DepositorInsertFlippase1::PEST
UseTagsPESTExpressionInsectMutationAspartic Acid at Amino Acid 5PromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with PA+Histag
Plasmid#84870PurposeBaculovirus Expression of Bacillus anthracis protective antigenDepositorInsertPA
UseBaculovirusTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3X-UASTattB
Plasmid#189859PurposeFly cloning and transformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4-vas2-integrase-R3
Plasmid#62299PurposephageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoterDepositorInsertphageC31 integrase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
UseTagsExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFB1.HMBP.PrS.SUZ12
Plasmid#125162PurposeExpresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein SUZ12 (SUZ12 Human)
UseBaculovirus expression vectorTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationE17K mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE2n
Plasmid#17456Purposemodified pENTR vector for N-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3x127D01-DsRed
Plasmid#171578PurposeFor scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE5c
Plasmid#17465Purposemodified pENTR vector for C-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
AlphaC
Plasmid#124525PurposepColE1_AlphaCDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACUH-sfcherry2(1-10)
Plasmid#172061PurposeUAS vector to express sfcherry2(1-10) in DrosophilaDepositorInsertsfcherry2(1-10)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
UseTags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)
Plasmid#172065PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intronDepositorInsertmTagBFP2(11x7)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCFD3-frame_selector_1
Plasmid#127554PurposeDrosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 1
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pFBD-SpyTag-noro-VLP
Plasmid#165989PurposeExpresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteinsDepositorInsertSpyTag-noro-VLP (ORF2 Synthetic, norovirus)
UseTagsSpyTagExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only