5,013 results

Showing: 931 - 960 of 5013 results

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
R979-X58-008: Hs.KRAS4b(1-188)

Plasmid

#159691

Purpose

Baculovirus protein expression of KRAS4b (1-188)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-188) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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IMPT-6213

Plasmid

#99333

Purpose

AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

Depositor

Raymond Stevens

Article

Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

Insert

AGTR2 (AGTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N116C-G193C

Plasmid

#162580

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Asparagine 116 was mutated to Cysteine and Glycin…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFB-HTB-ASK1

Plasmid

#29517

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

ASK1 (SKP1 Mustard Weed)

Expression

Insect

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCaST-elav-VP16AD

Plasmid

#15308

Depositor

Benjamin White

Article

Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

Insert

VP16AD-ZIP+

Expression

Insect

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only

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UAS-Halo7::CAAX

Plasmid

#87645

Purpose

For creating drosophila transgenics expressing UAS-Halo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Halo7::CAAX

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC153-20XUAS-IVS-B2::PEST

Plasmid

#32134

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pUAS-ERK-YFP

Plasmid

#42076

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila ERK ortholog (rl Fly)

Tags

YFP

Expression

Insect

Promoter

Hsp70

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pActin5c-CD-dCas9m4-UDI-attB

Plasmid

#104879

Purpose

To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

Depositor

Christopher Potter

Insert

CD-dCas9(m4)-UDI

Use

CRISPR

Expression

Insect

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pUASTB

Plasmid

#18944

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 attB

Expression

Insect

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA2

Plasmid

#115116

Purpose

Bait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G17230 X074_pECIA2

Plasmid

#115096

Purpose

Bait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17230 (AT1G17230 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G33170 X049_pECIA2

Plasmid

#115101

Purpose

Bait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G74360 X038_pECIA2

Plasmid

#115080

Purpose

Bait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDest-636

Plasmid

#159574

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Type

Empty backbone

Tags

His6-MBP

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA14

Plasmid

#114916

Purpose

Prey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT1G17230 X074_pECIA14

Plasmid

#114896

Purpose

Prey vector AT1G17230 X074_pECIA14 should be used with bait vector AT1G17230 X074_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17230 (AT1G17230 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G33170 X049_pECIA14

Plasmid

#114901

Purpose

Prey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT1G74360 X038_pECIA14

Plasmid

#114880

Purpose

Prey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pattB-synaptobrevin-GAL4-hsp70

Plasmid

#46107

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-3xHA-V5-RpL10Ab

Plasmid

#125223

Purpose

3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

3x HA, V5

Expression

Insect

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

Plasmid

#52890

Purpose

"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Inserts

LexGAD
Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217513

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pFastBacI-KDAC6

Plasmid

#224346

Purpose

Expresses human KDAC6 (HDAC6) in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacDual with LFn + Needle

Plasmid

#84868

Purpose

Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Needle

Use

Baculovirus

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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