5,017 results

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57F03_LexA-GAD

Plasmid

#83256

Purpose

Expresses LexA-GAD under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Expression

Insect

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC165-20XUAS-IVS-R::PEST

Plasmid

#32142

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

R::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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PPT1

Plasmid

#25205

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PPT1_02 (PPT1 Human)

Tags

His

Expression

Insect

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

Plasmid

#162384

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Human IntS6

Plasmid

#198408

Purpose

Expresses FLAG-tagged human IntS6

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

DDX26 (INTS6 Human)

Tags

3x FLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-MCS

Plasmid

#52908

Purpose

Aedes aegypti polyubiquitin promoter followed by MCS

Depositor

Zach Adelman

Type

Empty backbone

Expression

Insect

Promoter

Ae aegptyi polyubiquitin

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pOCC305

Plasmid

#118898

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease and TwinStrep-MB…

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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UAS-u(M)Cas9

Plasmid

#127384

Purpose

Expresses Cas9 at medium level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(M)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFast-Flag-hHus1

Plasmid

#222470

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Tags

Flag epitope

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_HT_B_6xHis-TEV-GFP-NBR1

Plasmid

#190928

Purpose

Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

NBR1 (NBR1 Human)

Tags

Strep-TEV-GFP

Expression

Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster nanos

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc V5 dDispatched WT HA

Plasmid

#126405

Purpose

Expression of V5 dDispatched WT HA in Drosophila cells lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE3-VP64-P10

Plasmid

#104607

Purpose

Expresses TALE3 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE3

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC4-3XUAS-IVS-mCD8::GFP

Plasmid

#26217

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Tags

myc

Expression

Insect

Promoter

tubulin

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCoofy51

Plasmid

#122005

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

TwinStrep

Expression

Insect

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINN-GFP

Plasmid

#53541

Purpose

pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

Depositor

Ray Owens

Insert

GFP

Tags

N-His-GFP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCoofy60

Plasmid

#122012

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-eGFP

Expression

Insect

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE4-VP64-P10

Plasmid

#104608

Purpose

Expresses TALE4 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE4

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-Actin5c

Plasmid

#15312

Depositor

Ron Vale

Article

Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

Insert

Actin5c (Act5C Fly)

Tags

EGFP and His

Expression

Insect

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only