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Plasmid#182205Purpose3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
DmU6 3p3 GFP LgRNA
Plasmid#182207PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in DrosophilaDepositorInsert3XP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-SAS6
Plasmid#80273Purposebaculovirus creation plasmid for GST-SAS6DepositorInsertSAS6 (SASS6 Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-syb-spCFP1-10
Plasmid#73699PurposeNeuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spCFP1–10 chimera
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G1
Plasmid#40174DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
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Hy_pMT EGFP SV40 pA
Plasmid#69911PurposeExpresses EGFP in DrosophilaDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-MS2_V
Plasmid#147737PurposeInsect Expression vectorDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S D808N
Plasmid#169947PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationD808NPromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac flag Bmi1
Plasmid#1967DepositorInsertBmi1 (Bmi1 Mouse)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQUAS-DSCP-FLPo
Plasmid#24358DepositorInsertFLPo
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoL-lexA
Plasmid#138404PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQL)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
R
Plasmid#167170PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cellsDepositorInsertsATP1A1-R
ATP1B1
UseTagsExpressionBacterial and InsectMutationPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPac0
Plasmid#12094DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSK41
Plasmid#74886Purposetemplate for insertion of attP site (C-terminal tag plasmid design), Puromycin-ResistanceDepositorInsertattP site
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)
Plasmid#169185PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab SARS-CoV-2, Synthetic)
UseTagsHis6-3xFlag (nsp12 C-terminus)ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_F2
Plasmid#171926PurposeBacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_F2
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac GST-Plk4
Plasmid#80264Purposebaculovirus creation plasmid for GST-Plk4DepositorInsertPlk4 (PLK4 Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC267
Plasmid#118887Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tagDepositorInsertNcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; HALO tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-Flag Ring1A
Plasmid#1995DepositorInsertRing1A (Ring1 Mouse)
UseTagsflagExpressionInsectMutationKozak before start codonPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
UAS-myr::4xCLIPf
Plasmid#87635PurposeFor creating drosophila transgenics expressing UAS-myr4x::CLIPfDepositorInsertmyr::4xCLIPf
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
Plasmid#87644PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2DepositorInsertmyr::SNAPf>myr::CLIPf>myr::Halo2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Or1a-Gal4
Plasmid#63019PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or1a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAEL003877-Cas9
Plasmid#100581PurposeThe promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL003877-hspcas9-GFP
Opie-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL003877 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
OA-1055J
Plasmid#200251PurposepBac-U6-gRNA(Intersex)-3xp3-tdTomatoDepositorInsert2 gRNAs targeting Intersex gene
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hy_pUbi-p63e eGFP SV40
Plasmid#132650PurposeExpresses eGFP mRNA from the Drosophila Ubi-p63e promoterDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterUbi-p63eAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac[6xP3-Tc-hsp-ECFP-SV40]
Plasmid#86447PurposepBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)DepositorInsert6xP3-Tc-hsp-ECFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
IL2 F42A pAcGP67a
Plasmid#41807DepositorInsertIL2 (IL2 Human)
UseTags6x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
p2X-UASTattB
Plasmid#189860PurposeFly cloning and transformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only