4,932 results

1. 5xGMR

   Plasmid

   #165775

   Purpose

   5xGMR syntethic eye driver enhancer, requires minimal promoter element

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   5xGMR
   

   Expression

   Insect

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAEL007584-Cas9

   Plasmid

   #100706

   Purpose

   The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL007584-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Promoter

   AAEL007584 and Opie2

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASp YFP Rab3 WT

   Plasmid

   #37687

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab3 (Rab3 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   Nov. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pUASp YFP Rab5 CA

   Plasmid

   #37702

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q88L (constitutive active)

   Promoter

   GAL4

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

5. Or45b-Gal4

   Plasmid

   #63036

   Purpose

   To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or45b, using the promoter sequences of the gene cloned 5' of Gal4

   Depositor

   Leslie Vosshall

   Article

   Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

   Insert

   Drosophila melanogaster Or45b promoter
   

   Tags

   Drosophila ADH (aa's 1-18) fused to Yeast Ga…

   Expression

   Insect

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

6. PI(LM)-Rac1(WT)dc1.FLARE

   Plasmid

   #87590

   Purpose

   PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   PBD
   Rac1
   

   Tags

   YPet and dTurq

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LIC4B-His-TEV-TAK1-TAB1

   Plasmid

   #154874

   Purpose

   Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

   Depositor

   Kenneth Westover

   Article

   Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

   Insert

   TAK1-TAB1 (TAB1 Human)
   

   Tags

   LIC TEV His

   Expression

   Insect

   Mutation

   Codon optimized

   Promoter

   polyhedrin

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR

   Plasmid

   #87642

   Purpose

   For creating drosophila transgenics expressing HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Bxb1
   

   Expression

   Insect

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

   Plasmid

   #172916

   Purpose

   Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7s-T2A-tdTomato
   

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVMG0212_Cq-nanos-Cas9

    Plasmid

    #169348

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0212_Cq-nanos-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ011551

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

    Plasmid

    #169370

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039596

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVMG0213_Cq-vasa-Cas9

    Plasmid

    #169347

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0213_Cq-vasa-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ009286

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pattB-nsyb-MKIIK11A::nlsLexADBDo

    Plasmid

    #64726

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKIIK11A::nlsLexADBDo
    

    Expression

    Insect

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAST YFP mouse Rab35 WT

    Plasmid

    #46014

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    Rab35 (Rab35 Mouse)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pFastBac-His6-Mps1-KD

    Plasmid

    #114064

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

    Insert

    Mps1-KD (TTK Human)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    Mps1 KD (D664A)

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pattB-3xFlag-Loqs-PD

    Plasmid

    #41110

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PD (loqs Fly)
    

    Tags

    3xFlag

    Expression

    Insect

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAC-7m1-QFBDADM1

    Plasmid

    #46097

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7m1
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXN-FBLWLF-GAL80

    Plasmid

    #26265

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac dRING1F

    Plasmid

    #1928

    Depositor

    Robert Kingston

    Article

    Francis et al Mol Cell 2001 Sep;8(3):545-56.

    Insert

    dRING1 (Sce Fly)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

20. 62 pLIB_N-MBP_C_FKBP

    Plasmid

    #160311

    Purpose

    N-term 3C cleavable MBP, C-term FKBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    6xHis, FKBP, and MBP

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169194

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    3xFlag-His6-PLpro (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAW-EGFP-2xmiR-1 (pAW-AS-21)

    Plasmid

    #40832

    Purpose

    miRNA sensor used in Drosophila cell lines

    Depositor

    Phillip Zamore

    Article

    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

    Insert

    Two target sites perfectly complementary to miR-1
    

    Use

    Mirna reporter

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pAC-luc-CLK

    Plasmid

    #33132

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

    Insert

    CLK 3' UTR (Clk Fly)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

24. ArpC2_pLib

    Plasmid

    #173679

    Purpose

    ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC2 (ARPC2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Hy_pMT Flag-UAP56 MUT

    Plasmid

    #110124

    Purpose

    Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    UAP56 (DDX39B Human)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KSLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUASp YFP Rab10 WT

    Plasmid

    #37708

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pUASp YFP Rab10 DN

    Plasmid

    #37709

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T23N (dominant negative)

    Promoter

    GAL4

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pBPGUw-HACK-QF2

    Plasmid

    #80276

    Purpose

    QF2 HACK backbone compatible with GMR attP integrated insertions.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBY2807

    Plasmid

    #23222

    Depositor

    Ralf Baumeister

    Article

    Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

    Insert

    Gateway-SnAvi
    

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pUASTattB-MKII::nlsLexADBDo

    Plasmid

    #64718

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKII::nlsLexADBDo
    

    Expression

    Insect

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUASP YFP Rab11 WT

    Plasmid

    #37711

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

32. BacPAK GST-Uch37N

    Plasmid

    #19421

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 1-239 (UCHL5 Human)
    

    Tags

    GST and His

    Expression

    Insect

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid

    #86456

    Purpose

    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

    Plasmid

    #131618

    Purpose

    Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM]
    NeoR[traF]
    

    Expression

    Insect

    Promoter

    Opie2

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Dll3ICD-HPC4 pBacPAK8-3

    Plasmid

    #17334

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Delta-like 3 (Dll3 Mouse)
    

    Tags

    HPC4

    Expression

    Insect

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

36. GFP-Rab30

    Plasmid

    #31743

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab30 (Rab30 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pUAST HRP Rab35 WT

    Plasmid

    #53498

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    Rab35 (Rab35 Fly)
    

    Tags

    HRP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid

    #169181

    Purpose

    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    No purification tag and nsp7-nsp8 fusion protein …

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDSAG

    Plasmid

    #62289

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. UAS-u(XL)Cas9

    Plasmid

    #127386

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XL)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge