4,931 results

1. pUASP dsRed Rab7 DN

   Plasmid

   #44826

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab7 (Rab7 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   T22N (dominant negative)

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. ADAP1

   Plasmid

   #163905

   Purpose

   Full length mouse ADAP1 for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-HT-Dmp53KRKR

   Plasmid

   #85699

   Purpose

   expression of Drosophila p53 KRKR mutant in Drosophila cells

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   Dmp53 (p53 Fly)
   

   Tags

   His

   Expression

   Insect

   Mutation

   K26R K302R

   Promoter

   Ac5

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pSBL_DK198

   Plasmid

   #156425

   Purpose

   Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (aa 42-275)
   

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   aa 42-275

   Promoter

   Polyhedrin

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1 Flag Su(Z)2

   Plasmid

   #20202

   Depositor

   Nicole Francis

   Article

   Lo et al Mol Cell Biol. 2009 Jan . 29(2):515-25.

   Insert

   Su(Z)2 (Su(z)2 Fly)
   

   Tags

   Flag

   Expression

   Insect

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pQF-ET

   Plasmid

   #24365

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QF
   

   Expression

   Insect

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xHA MED10 pBacPAK8

   Plasmid

   #17321

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   MED10 (Med10 Mouse)
   

   Tags

   2xHA

   Expression

   Insect

   Available Since

   March 11, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pAGW-dpse-GAL4-DBD

   Plasmid

   #125153

   Purpose

   Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
   

   Use

   Gateway fly expression vector

   Expression

   Insect

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSGE_91_4xUAS_dCP

   Plasmid

   #71169

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   91_4xUAS_dCP
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Promoter

   DSCP Drosophila synthetic core promoter

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3039 pVL E6-AP

    Plasmid

    #8646

    Depositor

    Peter Howley

    Article

    Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

    Insert

    E6-AP (UBE3A Human)
    

    Expression

    Insect

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pUAST tdTomato fascin WT

    Plasmid

    #53508

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    fascin (sn Fly)
    

    Tags

    tdtomato

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AlphaE

    Plasmid

    #124527

    Purpose

    pColE1_AlphaE

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hy_pPepck1 eGFP SV40

    Plasmid

    #132644

    Purpose

    Expresses eGFP mRNA from the Drosophila Pepck1 promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Expression

    Insect

    Promoter

    Pepck1

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R989-X27-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

    Plasmid

    #159554

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    G12C

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-15-LexABD-G4MD-QFAD

    Plasmid

    #46105

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexABD-G4MD-QFAD
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 45 pLIB_C_12HIS

    Plasmid

    #160271

    Purpose

    C-term uncleavable 12xHis tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 46 pLIB_3C_C_HIS

    Plasmid

    #160188

    Purpose

    C-term 3C cleavable His tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRB38

    Plasmid

    #72864

    Purpose

    template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    Strep - His8 - Strep -TEV tag
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUASt-ssBiP-ESCargo

    Plasmid

    #140166

    Purpose

    Expresses a regulatable secretory cargo for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Expression

    Insect

    Promoter

    hs70

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Expression

    Insect

    Promoter

    hs70

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJC-TALE-Kr

    Plasmid

    #46146

    Depositor

    David Stern

    Article

    Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

    Type

    Empty backbone

    Tags

    Kruppel repression domain

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 61 pLIB_N-Strep_C_FKBP

    Plasmid

    #160310

    Purpose

    N-term 3C cleavable 2xStrepII, C-term FKBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII and FKBP

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 60 pLIB_HIS_MYC

    Plasmid

    #160309

    Purpose

    N-term 3C cleavable His-Myc tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis and 6xMyc

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

    Plasmid

    #79031

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUWR-Cad-mTFP

    Plasmid

    #58327

    Purpose

    Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-mTFP
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

26. UAS-akkordion dNhe

    Plasmid

    #41976

    Depositor

    Norbert Perrimon

    Article

    Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

    Insert

    DRhoGEF2 (RhoGEF2 Fly)
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pFastBac K-M33-Flag

    Plasmid

    #1993

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pCaSpeR-act5cB-LexAQFw

    Plasmid

    #61311

    Purpose

    Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA_DBD::QF2w_AD
    

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDSARN

    Plasmid

    #62295

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKM568

    Plasmid

    #134217

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C
    

    Expression

    Insect

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJC-ase0.8kb-TALE3-VP64-P10

    Plasmid

    #104610

    Purpose

    Expresses TALE3 under the control of a 0.8kb ase enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    0.8kb ase enhancer element (ase Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUAST Ag83b

    Plasmid

    #53443

    Purpose

    UAS-Anopheles gambiae Orco in pUAST Vector

    Depositor

    Leslie Vosshall

    Insert

    pUAST Ag83b (NEWENTRY Anopheles gambiae)
    

    Expression

    Insect

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

33. Act5C-pDONR221

    Plasmid

    #124242

    Purpose

    Gateway entry clone of Drosophila Actin5C cDNA.

    Depositor

    Peter Vilmos

    Insert

    Drosophila Actin 5C (Act5C Fly)
    

    Expression

    Insect

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. R725-X09-635: His6-tev-Hs.SPRED1(13-125)

    Plasmid

    #159581

    Purpose

    Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQF-M2ET

    Plasmid

    #24364

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QF
    

    Expression

    Insect

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUAS-hopscotch

    Plasmid

    #37301

    Depositor

    Norbert Perrimon

    Article

    Harrison et al EMBO J. 1995 Jun 15;14(12):2857-65.

    Insert

    hopscotch (hop Fly)
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

37. CJ19 (miR-1006 minigene)

    Plasmid

    #17760

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1006 minigene
    

    Use

    Drosophila metallothionein gene promoter drives e…

    Expression

    Insect

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

38. U6a-T

    Plasmid

    #117209

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    u6a promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUASp YFP Rab10 CA

    Plasmid

    #37710

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q68L (constitutive active)

    Promoter

    GAL4

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

40. 908E

    Plasmid

    #160238

    Purpose

    TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Ttg Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge