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pFastBac HTB DPP9S

Plasmid

#166787

Purpose

Insect cell expression vector for DPP9S (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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ORCO-T2A-QF-DBD

Plasmid

#162525

Purpose

Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

Depositor

Leslie Vosshall

Article

Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

Insert

Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP3

Plasmid

#178069

Purpose

Insect cell expression of GCP3

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP3 (TUBGCP3 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-WT

Plasmid

#114063

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-WT (TTK Human)

Use

Tags

His6

Expression

Insect

Mutation

none

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-blast

Plasmid

#208034

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-LexA

Plasmid

#26266

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pKSB-611U6-sgRNA3 AGAG AACA

Plasmid

#173673

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2-VHH(GFP)

Plasmid

#126022

Purpose

express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pattB-QF-sv40

Plasmid

#24367

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

QF

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pACU

Plasmid

#58373

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-7m1-QFBDADM1-hsp70

Plasmid

#46116

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBac{Mhc-453-GFP,attB,w+}

Plasmid

#182199

Purpose

EGFP driven by 453bp enhancer of Mhc gene

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 4x

Plasmid

#20930

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Mar 4. ():.

Insert

4x let-7 target sites

Use

Luciferase

Tags

Expression

Insect and Mammalian

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

copia

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Rab18

Plasmid

#31740

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab18 (Rab18 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pNos-PE2-attB

Plasmid

#149549

Purpose

Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

nanos (nos)

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_641-1574_AH

Plasmid

#148920

Purpose

Insect Expression of DmGIGYF_641-1574

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_641-1574 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_1-640_AH

Plasmid

#148919

Purpose

Insect Expression of DmGIGYF_1-640

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_1-640 (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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