4,908 results

1. pUAS-kkv::NG

   Plasmid

   #138953

   Purpose

   Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4

   Depositor

   Paul Adler

   Article

   Adler et al bioRxiv 2020

   Insert

   kkv (NEWENTRY Fly)
   

   Use

   Uas transgene vector

   Tags

   Neon Green fused to C terminus of Kkv

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. OA-1067K

   Plasmid

   #164848

   Purpose

   Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

   Depositor

   Omar Akbari

   Article

   Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

   Insert

   4 gRNAs and 3xP3-tdTomato
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac GST-SAS6

   Plasmid

   #80273

   Purpose

   baculovirus creation plasmid for GST-SAS6

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   SAS6 (SASS6 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriExEKAR2G1

   Plasmid

   #40174

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. OA-874PA

   Plasmid

   #164846

   Purpose

   Expresses AAEL005635-Cas9 with OpIE2-CFP marker

   Depositor

   Omar Akbari

   Article

   Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

   Insert

   P10-3'UTR OpIE2-CFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSTARR-seq_fly-RpS12

   Plasmid

   #71504

   Purpose

   flySTARR-seq screening vector with RpS12 core promoter

   Depositor

   Alexander Stark

   Article

   Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINP

   Plasmid

   #41139

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ac5-STABLE1-puro

   Plasmid

   #208031

   Purpose

   Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2Bac.Myc.p44

   Plasmid

   #39220

   Depositor

   Aziz Sancar

   Insert

   p44 (GTF2H2 )
   

   Use

   Tags

   Myc

   Expression

   Insect

   Mutation

   Promoter

   Pp10

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pFastBac1-Nucleocapsid

    Plasmid

    #179696

    Purpose

    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

    Depositor

    Mark Arbing

    Insert

    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His6-TEV protease site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMT EGFP SV40 pA

    Plasmid

    #69911

    Purpose

    Expresses EGFP in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQUAST-syb-spCFP1-10

    Plasmid

    #73699

    Purpose

    Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spCFP1–10 chimera
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT Lifeact-TagBFP

    Plasmid

    #126664

    Purpose

    Inducible expression of Lifeact-TagBFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Lifeact-TagBFP fusion
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CapChR2_pUAST-attb

    Plasmid

    #188041

    Purpose

    Expresses CapChR2 in Drosophila

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2
    

    Use

    Tags

    none

    Expression

    Insect

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    UAS

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid

    #157813

    Purpose

    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

    Depositor

    Zoltan Lipinszki

    Article

    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

    Insert

    GFP-binding protein
    

    Use

    For drosophila cells

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    Promoter

    metallothionein

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. gp64-mVEGF-C-HisTag-pENTR

    Plasmid

    #64065

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFD3-Act5c

    Plasmid

    #130278

    Purpose

    Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    pCFD3-Act5c (Act5C Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. OA-961B

    Plasmid

    #104967

    Purpose

    D. suzukii Medea Construct

    Depositor

    Omar Akbari

    Article

    Buchman et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Inserts

    GFP
    dsRed
    HR5Ie1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAFMW-AthHEN1

    Plasmid

    #104980

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-Rab10-FLAG

    Plasmid

    #135559

    Purpose

    Express mouse Rab10 in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge