5,046 results
-
Plasmid#146621PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pAc5.1B-V5-SBP_J
Plasmid#146633PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Flp1)
Plasmid#194770PurposeTo insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.DepositorInsertT2A-Flp1
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-Fml1
Plasmid#193058PurposeBaculovirus expression of S.pombe Fml1 for recombinant protein productionDepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
TagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-6His-Nter-GWs-Lox (VE5587)
Plasmid#163768PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.DepositorInsertN-terminal 6His tag
Tags6 His TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-10His-Cter (VE5631)
Plasmid#161802PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.DepositorInsertC-terminal 10 His tag
Tags10 HisExpressionInsectPromoterp10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-10His-Cter (VE5742)
Plasmid#139776PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.DepositorInsertC-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 WWE-PARP
Plasmid#192550PurposeE. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mammā¦PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
TagsAcGFP1ExpressionInsectPromoterAc5Available SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P10.1-StrepTagII
Plasmid#191475PurposeExpresses K2P10.1 with C-terminal StrepTagIIDepositorExpressionInsectMutationN144Q (uniprot P57789-1)PromoterpolHAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3X-UASTattB
Plasmid#189859PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2X-UASTattB
Plasmid#189860PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-mC-S
Plasmid#172428PurposeExpression of full-length, human GIRK4 with C-terminal mCherry StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_2
Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1053A
Plasmid#184006PurposeExpresses arrays of gRNA targeting eve promoter under U6b/c promoterDepositorInsertU6b:sgRNAeve1 - U6c:sgRNAeve2
ExpressionInsectAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF2
Plasmid#162524PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
ExpressionInsectAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-DDIT4
Plasmid#187116Purposeinsect cell expression of DDIT4DepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfCsf1a
Plasmid#168103PurposeExpress soluble His-tagged zebrafish Csf1a in insect cellsDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX4
Plasmid#183903Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
ExpressionInsectPromoterAedes aegypti PUbAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX3
Plasmid#183904Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
ExpressionInsectPromoterAedes aegypti PolyubiquitinAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H
Plasmid#146503PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
ExpressionInsectAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Clover4-LUMABS-S1 RBD
Plasmid#183491PurposeTo express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.DepositorInsertClover4-LUMABS-S1 RBD
ExpressionInsectAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)
Plasmid#185448PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cellsDepositorTagsMBP, YBBR, and ZZExpressionInsectMutationSee Depositor Comments BelowAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF1
Plasmid#185445PurposeExpresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK
Plasmid#186141PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_1-640_AH
Plasmid#148919PurposeInsect Expression of DmGIGYF_1-640DepositorInsertDmGIGYF_1-640 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence giveā¦Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-6His-Cter (VE5630)
Plasmid#139777PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.DepositorInsertC-terminal 6His tag
Tags6 HisExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only