4,908 results

1. pFastBac GST-Plk4

   Plasmid

   #80264

   Purpose

   baculovirus creation plasmid for GST-Plk4

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQUAS-DSCP-FLPo

   Plasmid

   #24358

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   FLPo
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINO_F2

   Plasmid

   #171926

   Purpose

   Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

   Insert

   VHH_F2
   

   Use

   Tags

   His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pFastBac(His10-Shp2)

   Plasmid

   #177899

   Purpose

   Baculo expression of His10 tag fused to human Shp2

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   Shp2 (PTPN11 Human)
   

   Use

   Tags

   10xHis-TEV-SNAP tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-MS2_V

   Plasmid

   #147737

   Purpose

   Insect Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASp YFP Rac1 DN

   Plasmid

   #52234

   Purpose

   Drosophila expression of YFP-Rac1 dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Rac1 (Rac1 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   T17N; DN mutation

   Promoter

   GAL4

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. rNrx1beta(47-299)

   Plasmid

   #20173

   Depositor

   Axel Brunger

   Article

   Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

   Insert

   Neurexin 1 beta (Nrxn1 Rat)
   

   Use

   Tags

   6X His tag

   Expression

   Insect

   Mutation

   no splice site

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. R

   Plasmid

   #167170

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac HTB DPP9S D808N

   Plasmid

   #169947

   Purpose

   Purification of catalytically-dead DPP9S from insect cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   D808N

   Promoter

   polyhedrin

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSK41

    Plasmid

    #74886

    Purpose

    template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    attP site
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAS-kkv-R896K::NG

    Plasmid

    #138957

    Purpose

    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv-R896K::NG (kkv Fly)
    

    Use

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    R896K

    Promoter

    UAS

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLib6.4-Aalb_744

    Plasmid

    #176663

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRB39

    Plasmid

    #72870

    Purpose

    template for N-terminal eGFP tag, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1 PIK3R1-Ni

    Plasmid

    #17744

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1-Ni (PIK3R1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Partial cDNA

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. LexAop2-myr::4xSNAPf

    Plasmid

    #87638

    Purpose

    For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xSNAPf
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hy_pUbi-p63e eGFP SV40

    Plasmid

    #132650

    Purpose

    Expresses eGFP mRNA from the Drosophila Ubi-p63e promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pRB40

    Plasmid

    #72865

    Purpose

    template for N-terminal eGFP tag, constitutive Blasticidin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Or1a-Gal4

    Plasmid

    #63019

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

    Insert

    Drosophila melanogaster Or1a promoter
    

    Use

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. U6-BbsI-crRNA

    Plasmid

    #51026

    Purpose

    Generates crRNA for use in combination with tracrRNA

    Depositor

    Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

    Insert

    U6-BbsI-crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-2

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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