4,908 results

1. pAct-dCBEevoAPOBEC1

   Plasmid

   #195514

   Purpose

   Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   dCBE-evoAPOBEC1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

   Plasmid

   #205152

   Purpose

   Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   FRT-GFP-FRT-uORF-dCBEevoCDA1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKM463

   Plasmid

   #134214

   Purpose

   Baculovirus expression of CENP-U and CENP-Q

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   his-CENP-U + Q
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBacPAK8-ElonginB

   Plasmid

   #29504

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

   Insert

   Elongin B (ELOB Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pAc5 dOrai

   Plasmid

   #16368

   Depositor

   Anjana Rao

   Article

   Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

   Insert

   Orai (olf186-F Fly)
   

   Use

   Tags

   V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pKM498

   Plasmid

   #134215

   Purpose

   Baculovirus expression of CENP-T and CENP-W

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   his-CENPT + CENPW
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EKAR2G_design1_mTFP_227_Venus_173

   Plasmid

   #39835

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBPHLWL

   Plasmid

   #26254

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   Multiple cloning site
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

   Plasmid

   #172918

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7c-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac FPC

    Plasmid

    #1927

    Depositor

    Robert Kingston

    Article

    Francis et al Mol Cell 2001 Sep;8(3):545-56.

    Insert

    Polycomb (Pc Fly)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid

    #145940

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (N45D and G558R) and thr…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIS1

    Plasmid

    #74887

    Purpose

    targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Inserts

    Act5C upstream homology region
    Act5C downstream homology
    

    Use

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAcGP67B MBP-tagged human Norrin ( 31–133)

    Plasmid

    #132625

    Purpose

    expresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

    Insert

    MBP-Norrin ( 31–133)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AeU6 3p3 GFP LgRNA

    Plasmid

    #182208

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

    Plasmid

    #32147

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    rox> STOP STOP rox>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFTSG

    Plasmid

    #162389

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HS-H3.3A-GFP

    Plasmid

    #8696

    Depositor

    Kami Ahmad

    Article

    Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

    Insert

    H3.3A (His3.3A Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 907L

    Plasmid

    #160237

    Purpose

    FlightN-ttgR-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    TtgR repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASTLOTattB_mCherry::Nrv1::vhhGFP4

    Plasmid

    #163926

    Purpose

    Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

    Plasmid

    #126027

    Purpose

    express Flag-tagged LARIAT for optogenetic clustering

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CIBN-MP; CRY2 PHR-VHH(GFP)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    WT

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge