4,908 results

1. pUAST-HA, Pbl-A

   Plasmid

   #69792

   Purpose

   Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

   Depositor

   Arno Muller

   Article

   van Impel et al Development. 2009 Mar;136(5):813-22. doi

   Insert

   Pebble - Drosophila guanine nucleotide exchange factor
   

   Use

   P element-based puast vector for gal4-regulated e…

   Tags

   HA tag

   Expression

   Insect

   Mutation

   Encodes Pbl (NP_729306.1) lacking last 9 amino ac…

   Promoter

   hsp70 promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMT-GFP-Sas4

   Plasmid

   #31756

   Depositor

   Ron Vale

   Article

   Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

   Insert

   Sas4 (Sas-4 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   N70T

   Promoter

   Metallothionein

   Available since

   Jan. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAC-luc-TimRA

   Plasmid

   #33133

   Depositor

   Michael Rosbash

   Article

   Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

   Insert

   Tim-RA 3' UTR (tim Fly)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Expression

   Insect

   Mutation

   Promoter

   Actin 5C

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMT-GFP-SAK

   Plasmid

   #31755

   Depositor

   Ron Vale

   Article

   Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

   Insert

   Sak (SAK Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   A115S

   Promoter

   Metallothionein

   Available since

   Jan. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. FAK

   Plasmid

   #186141

   Purpose

   For expresseion of recombinant FAK using baccolovirus in SF9 cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PTK2 (PTK2 Mouse)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 59 pLIB_HIS_HA

   Plasmid

   #160308

   Purpose

   N-term 3C cleavable His-HA tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   12xHis and 3xHA

   Expression

   Insect

   Mutation

   Promoter

   polyhydrin

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-HA-STX4

   Plasmid

   #89311

   Purpose

   Expresses HA tagged syntaxin 4 in Drosophila cells

   Depositor

   Andrew Peden

   Insert

   STX4 (Syx4 Fly)
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   Promoter

   P-MT (Metallothionein)

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAGHis10pFastBacHTA/Serca1

   Plasmid

   #74401

   Purpose

   expression of SERCA in insect cells

   Depositor

   Robert Nakamoto

   Insert

   SERCA1 (ATP2A1 rabbit)
   

   Use

   Tags

   10His and FLAG

   Expression

   Insect

   Mutation

   NcoI site destroyed (silent) T898

   Promoter

   polyhedrin

   Available since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pUAST tdTomato mouse Rab35 WT

   Plasmid

   #53514

   Depositor

   Matthew Scott

   Article

   Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

   Insert

   Rab35 (Rab35 Mouse)
   

   Use

   Tags

   tdtomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCaSpeR4 cyto Grx1-roGFP2

    Plasmid

    #64999

    Purpose

    Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFRC7-RFPnls-PQRv2-mCD8:GFP

    Plasmid

    #73974

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP
    RFP
    

    Use

    Tags

    mCD8 and nls

    Expression

    Insect

    Mutation

    Promoter

    20XUAS-HS

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBC2FG

    Plasmid

    #31281

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Cry2 (Cry2 Mouse)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pUAST-FOXO 3A

    Plasmid

    #52983

    Purpose

    insect expression of FOXO triple mutant

    Depositor

    Marc Montminy, Biao Wang

    Insert

    FOXO (foxo Fly)
    

    Use

    Tags

    3x HA

    Expression

    Insect

    Mutation

    3A, triple mutant, T44A S190A S259A

    Promoter

    Hsp70

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSGE_763_4xUAS_UPS_hkCP

    Plasmid

    #71171

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    763_4xUAS_UPS_hkCP
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    DSCP Drosophila synthetic core promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

    Plasmid

    #86455

    Purpose

    Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p5xUAS

    Plasmid

    #165891

    Purpose

    Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    5xUAS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAL80

    Plasmid

    #165825

    Purpose

    GAL4 inhibitor coding sequence (CDS)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GAL80
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQS

    Plasmid

    #165826

    Purpose

    QF inhibitor coding sequence (CDS)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    QS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p5xQUAS

    Plasmid

    #165818

    Purpose

    Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    5xQUAS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac GST-STIL

    Plasmid

    #80265

    Purpose

    baculovirus creation plasmid for GST-STIL

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge