4,908 results

1. pBacPAK8-VHL

   Plasmid

   #29498

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

   Insert

   Von Hippel-Lindau (VHL Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pUASp YFP Rab35 DN

   Plasmid

   #53495

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab35 (Rab35 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   S22N

   Promoter

   GAL4

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Ir25a_GAL80

   Plasmid

   #83259

   Purpose

   Expresses GAL80 under the control of Ir25a enhancer

   Depositor

   Leslie Vosshall

   Article

   Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

   Insert

   Ir25a enhancer
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Or42a-Gal4

   Plasmid

   #63032

   Purpose

   To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or42a, using the promoter sequences of the gene cloned 5' of Gal4

   Depositor

   Leslie Vosshall

   Article

   Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

   Insert

   Drosophila melanogaster Or42a promoter
   

   Use

   Tags

   Drosophila ADH (aa's 1-18) fused to Yeast Ga…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDSAT

   Plasmid

   #62290

   Purpose

   docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCFD3-MS2tail-Scaffold

   Plasmid

   #78906

   Purpose

   Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UAS-<FRT.STOP<Bxb1

   Plasmid

   #87643

   Purpose

   For creating drosophila transgenics expressing UAS-<FRT.STOP<Bxb1

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Bxb1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUAST YFP mouse Rab35 DN

   Plasmid

   #46016

   Depositor

   Matthew Scott

   Article

   Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

   Insert

   Rab35 (Rab35 Mouse)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   S22N (dominant negative)

   Promoter

   GAL4

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pOCC215

   Plasmid

   #118879

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   Dendra2, HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAS-CFP-MEK

    Plasmid

    #42077

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila MEK ortholog (Dsor1 Fly)
    

    Use

    Tags

    CFP

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pUASp YFP Rholike DN

    Plasmid

    #52240

    Purpose

    Drosophila expression of YFP-Rholike dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rholike (RhoL Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T25N; DN mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pF3BGX-Venus

    Plasmid

    #138388

    Purpose

    RMCE vector for generating C-terminal Venus fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    Venus
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Opie2-PuroR[traF]

    Plasmid

    #131615

    Purpose

    Expresses a functional PuroR only in females and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Insert

    PuroR[traF]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcUW31-M10.5/human b2m

    Plasmid

    #11521

    Depositor

    Pamela Bjorkman

    Article

    Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

    Insert

    M10.5 (H2-M10.4 Mouse)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRB31

    Plasmid

    #72868

    Purpose

    template for N-terminal 1x Strep tag, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    single Strep tag
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EPHA2 (3FL7)

    Plasmid

    #25355

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    epha2.023.531 (EPHA2 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 19, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pRB30

    Plasmid

    #72860

    Purpose

    template for N-terminal eGFP tag, inducible Blasticidin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    eGFP

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT Draper-ITAM only-GFP

    Plasmid

    #126656

    Purpose

    Inducible expression of Draper-ITAM only-GFP mutant in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Cytoplasmic ITAM residues Tyr all others mutated …

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC207

    Plasmid

    #118869

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mCherry

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASP YFP Rab7 WT

    Plasmid

    #37699

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only