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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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82,463 results

Showing: 2401 - 2420 of 82463 results

  1. mEmerald-Sec61b-C1

    Plasmid
    #90992
    Purpose
    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEmerald-Sec61b (SEC61B Human, Synthetic)
    Tags
    mEmerald (green fluorescence)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    90992-plasmid-map-sequence-id-179977

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  3. pICE-HA-MRE11-WT

    Plasmid
    #82033
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    82033-plasmid-map-sequence-id-490398

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  5. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Publication
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    Rep2
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    68837-plasmid-map-sequence-id-121219

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  16. pCMV-GenEPi

    Plasmid
    #140236
    Purpose
    Mammalian expression of Piezo1-based fluorescent force sensor GenEPi
    Depositor
    Periklis Pantazis
    Publication
    Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
    Tags
    GCaMP 6s RS1 EF4
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    140236-plasmid-map-sequence-id-308737

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  19. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid
    #141379
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    13 Articles
    141379-plasmid-map-sequence-id-288091

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  20. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    140002-plasmid-map-sequence-id-275516

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Showing: 2401 - 2420 of 82463 results