78,002 results

1. pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

   Plasmid

   #169325

   Purpose

   Expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKrox24(2xD-E_inD)Luc

   Plasmid

   #200109

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element in front of EGR1 promoter…

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti SYN-FLAG-dCas9-VPR

   Plasmid

   #114196

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SYN

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-opto-hCaspase-9

   Plasmid

   #208787

   Purpose

   Inducibly expresses human Caspase 8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Caspase 9 (CASP9 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-lyso-pHluorin

   Plasmid

   #70113

   Purpose

   Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
   

   Tags

   HA-Tag and pHluorin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-dTAG-Blast

   Plasmid

   #207716

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. FLAG-Pol-II WT

   Plasmid

   #35175

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-TREX1

   Plasmid

   #27219

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. G5-CD40

   Plasmid

   #121166

   Purpose

   pMP71 vector expressing GFP - P2A - G5 - myc - CD40

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   G5-CD40
   

   Use

   Retroviral

   Tags

   GFP and myc

   Expression

   Mammalian

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human Beta-catenin GFP

    Plasmid

    #71367

    Purpose

    mammalian expression of human Beta-catenin GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    Beta-catenin (CTNNB1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-myc-FLNa WT

    Plasmid

    #8982

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A (FLNA Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid

    #177328

    Purpose

    Control vector for Flp-dependent DREADD constructs

    Depositor

    Ulrik Gether

    Insert

    mCherry
    

    Use

    AAV; Flp-frt

    Expression

    Mammalian

    Promoter

    Human Synapsin-1

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRUPATH Triple GaoA

    Plasmid

    #196051

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid

    #210656

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Anti-HA [12CA5] mouse IgG2a heavy chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse IgG2a heavy chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTIPI_mK_Anti-HA [12CA5]

    Plasmid

    #210657

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Anti-HA [12CA5] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMito-MtrxRFP2

    Plasmid

    #172324

    Purpose

    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SNCA-A53T-mRuby3

    Plasmid

    #215379

    Purpose

    Expression of the A53T mutant of alpha-synuclein with a C-terminal mRuby3 tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Tags

    mRuby3 tag

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-VSVN

    Plasmid

    #64087

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV nucleoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG5-BZLF1

    Plasmid

    #72637

    Purpose

    Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BZLF1
    

    Expression

    Mammalian

    Mutation

    Q163R

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only