78,002 results

1. C-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58694

   Purpose

   Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Csplit Intein and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-CEACAM1-COMP5AP-AviTag-9xHis

   Plasmid

   #157390

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CEACAM1 (CEACAM1 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-ORF-V2

   Plasmid

   #73638

   Purpose

   Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-Lifeact-7

   Plasmid

   #54674

   Purpose

   Localization: Actin, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   LifeAct-mRuby2

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCMV- δLight

   Plasmid

   #201224

   Purpose

   Express deltaLight biosensor in mammalian cells

   Depositor

   Lin Tian

   Article

   Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

   Insert

   δLight1.3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdTomato-H2B-6

   Plasmid

   #58101

   Purpose

   Localization: Nucleus/Histones, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   H2B (H2BC11 Human)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaVα2δ1

   Plasmid

   #26575

   Depositor

   Diane Lipscombe

   Article

   Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

   Insert

   Cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK287 (mAID-Hygro)

   Plasmid

   #72825

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Hygro
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKeima-LiveDrop

   Plasmid

   #199695

   Purpose

   lipophagy reporter system based on the Keima-fluorophore

   Depositor

   Tobias Walther

   Article

   Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

   Insert

   GPAT4 (Gpat4 Fly)
   

   Tags

   Keima

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHalo-MyosinIIB-C25

    Plasmid

    #128574

    Purpose

    Studies of cellular contractility.

    Depositor

    Dylan Burnette

    Article

    Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

    Insert

    Myosin IIB (MYH10 Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pCMV R-CEPIA3mt

    Plasmid

    #140464

    Purpose

    Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

    Insert

    R-CEPIA3mt
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SARS2SΔC-H2gp41

    Plasmid

    #170389

    Purpose

    Generation of SARS-CoV-2 spike pseudotyped lentivirus

    Depositor

    Nir Hacohen

    Article

    Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Tags

    HIV gp41

    Expression

    Mammalian

    Mutation

    Deletion of C terminal and fusion with H2 from HI…

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Pam-FL

    Plasmid

    #42570

    Depositor

    Vijaya Ramesh

    Article

    Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

    Insert

    human MYCBP2 (MYCBP2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Pam-FL clone lacks alternatively spliced exons 52…

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE3G-EGFP-IRES(Neo)R

    Plasmid

    #158068

    Purpose

    eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    eGFP
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCDH-WT-ALKBH5-3xFLAG

    Plasmid

    #203736

    Purpose

    Overexpression of ALKBH5-WT

    Depositor

    Jianjun Chen

    Article

    Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

    Insert

    ALKBH5 (ALKBH5 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-mhYFP

    Plasmid

    #186533

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTripz Sox4.965

    Plasmid

    #101120

    Purpose

    shRNA Vector

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    Sox4.965
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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