77,980 results

1. CABE-T2.9

   Plasmid

   #193264

   Purpose

   Expression of CABE-T2.9 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.9
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA HA HP1 alpha

   Plasmid

   #24078

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 alpha (CBX5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA4-TO-Puromycin-mVenus-MAP

   Plasmid

   #44118

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Tags

   mVenus-MAP

   Expression

   Mammalian

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Expression

   Mammalian

   Promoter

   CAG and Human U6

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Bxb1-LP-v2-TC

   Plasmid

   #194326

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

   Plasmid

   #216494

   Purpose

   Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAktKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. MYC-hTRAK2

   Plasmid

   #225142

   Purpose

   Expresses MYC-tagged human TRAK2

   Depositor

   Thomas Schwarz

   Article

   Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

   Insert

   TRAK2 (TRAK2 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tag5Amyc-GSK3b KD

   Plasmid

   #16262

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Kinase dead: K85A

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Frankenbody-FLAG-Halo

   Plasmid

   #175296

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with HaloTag
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Tags

    mito

    Expression

    Mammalian

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-hPIKfyve

    Plasmid

    #121148

    Purpose

    Expresses GFP-tagged human PIKfyve in mammalian cells.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

    Insert

    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-rab11 WT

    Plasmid

    #12674

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDule2-Mb haloTyrRS C6

    Plasmid

    #160378

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-TLR9-YFP

    Plasmid

    #13642

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 9 (TLR9 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Neo-Bam

    Plasmid

    #16440

    Purpose

    Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE LOX Cloning template vector

    Plasmid

    #139651

    Purpose

    Cloning template for ORANGE-CAKE conditional knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIPZ

    Plasmid

    #206981

    Purpose

    empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Connexin43-GFP-APEX2

    Plasmid

    #49385

    Purpose

    Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Connexin43 - EGFP - APEX2
    

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myc-E-Syt1

    Plasmid

    #66833

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. EBFP2-H2B-6

    Plasmid

    #55243

    Purpose

    Localization: Nucleus/Histones, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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