77,958 results

1. pMRX-IP-EGFP-mouse Plaat3 (WT)

   Plasmid

   #167987

   Purpose

   Expresses EGFP-Plaat3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Hrasls3 (Plaat3 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR'-NRSF-CITE-GFP

   Plasmid

   #21310

   Depositor

   Jay Nadeau

   Article

   Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

   Insert

   neuron restrictive silencer factor (Rest Mouse)
   

   Use

   Lentiviral

   Tags

   GFP (bicistronic with CITE/IRES)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Oct. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. enIscB-T5E

   Plasmid

   #205411

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
   

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ADORA2A-Tango

   Plasmid

   #66210

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADORA2A (ADORA2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. mCherry-Calreticulin-N-16

   Plasmid

   #55006

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Calreticulin (CALR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-EB3mCherry_cmv-LifeActGFP

   Plasmid

   #133927

   Purpose

   Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-4EBP1 F113A

   Plasmid

   #81122

   Purpose

   expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

   Insert

   4EBP1 F113A (Eif4ebp1 Mouse)
   

   Expression

   Mammalian

   Mutation

   F113A mutation

   Promoter

   CAG

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE reporter backbone

    Plasmid

    #158678

    Purpose

    This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100854

    Purpose

    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jRGECO1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEG BacMam N term His8 eGFP 3C

    Plasmid

    #160684

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    His8 eGFP 3C

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-mEGFP(A206K)-paCaMKII(K42M)

    Plasmid

    #165440

    Purpose

    Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)
    

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Tags

    EGFP and His6

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCbs FlagOmomyc

    Plasmid

    #113168

    Purpose

    Expression of FLAG tagged Omomyc in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

    Insert

    Omomyc (MYC Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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