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CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

Plasmid

#107307

Purpose

Encoding an SPH activation system

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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MMLV-RT

Plasmid

#181801

Purpose

MMLV-RT (3XFLAG-SV40-MMLVRT)

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-HaloCaMP1a-EGFP

Plasmid

#138327

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has Service

AAV1 and AAV9

Insert

HaloCaMP1a

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Promoter

synapsin

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPAmCherry2-C1

Plasmid

#31932

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry2

Expression

Mammalian

Mutation

M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-UBR5

Plasmid

#52050

Purpose

CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

Depositor

Darren Saunders

Article

Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

Insert

UBR5 (UBR5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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MGAT1-GFP

Plasmid

#163647

Purpose

Expresses GFP tagged MGAT1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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OptoEphB2-mCherry

Plasmid

#92042

Purpose

Expresses OptoEphB2 labeled with mCherry

Depositor

Ji Yu

Insert

EPHB2 (EPHB2, CRY2 Human, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-PNKP

Plasmid

#215137

Purpose

Expresses mCherry-PNKP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PNKP (PNKP Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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Puro.Cre empty vector

Plasmid

#17408

Purpose

Lentiviral expression of Cre from the mouse PGK promoter

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

mouse PGK

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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huCaV2.2 (pSAD442-1)

Plasmid

#62574

Purpose

Human CaV2.2 expression in mammalian cells

Depositor

Diane Lipscombe

Article

Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

Insert

CACNA1B (CACNA1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158740

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/MI/45/15 H1N1

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ14566

Plasmid

#239272

Purpose

Expresses TRF1-PYL1 for recruiting RNA to the telomere via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

TRF1-BFP-PYL1 (TERF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10539

Plasmid

#239270

Purpose

Expresses PYL1-DDX6 for recruiting RNA to the p-body via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PYL1-BFP-DDX6 (DDX6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10531

Plasmid

#239267

Purpose

Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

NLS-dPspCas13b-EGFP-ABI

Use

CRISPR and Lentiviral

Tags

Flag tag and V5 tag

Expression

Mammalian

Mutation

H133A and H1058A in PspCas13b

Promoter

EF1a

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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GRM5-DuET

Plasmid

#213303

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GRM5 (GRM5 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC7

Plasmid

#231656

Purpose

Expresses human ZDHHC7 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC7 (ZDHHC7 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-TurboID-MCS

Plasmid

#186813

Purpose

CAG promoter-driven expression of TurboID-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

3xFLAG-TurboID

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJAF205

Plasmid

#60407

Purpose

Flag-tagged full length GMAP210/Trip11

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

Insert

GMAP210 (Trip11 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-MMLVgag-D3A-3L-dCas9-KOX1

Plasmid

#240532

Purpose

Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-D3A-3L-dCas9-KOX1

Tags

FLAG, HA

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0692

Plasmid

#143064

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HNF4A (HNF4A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-IRES-HO-1

Plasmid

#80345

Purpose

Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163057

Purpose

Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Purpose

Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-BEACON2

Plasmid

#171698

Purpose

Expresses BEACON2 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

Insert

BEACON2

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_W98Y/W104A/Y130F

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Neo

Plasmid

#99378

Purpose

Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

hNGN2-P2A-Neo (NEUROG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24

Plasmid

#28138

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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