78,002 results

1. scAAV-sgRNA-GFP

   Plasmid

   #177935

   Purpose

   Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD109-bio-His

   Plasmid

   #51862

   Purpose

   Expresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD109 (CD109 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2 TDP-43 NLS1 YFP

   Plasmid

   #84912

   Purpose

   Mammalian expresion of TDP-43 NLS1 YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TODestFLAGHA_HuD

   Plasmid

   #65757

   Purpose

   expresses FLAGHA-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kaede-N1

   Plasmid

   #54726

   Purpose

   Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Type

   Empty backbone

   Tags

   Kaede

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-DsRed

   Plasmid

   #11151

   Purpose

   Mammalian expression vector for expression of DsRed

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   DsRed2 from Discosoma sp
   

   Expression

   Mammalian

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. CP1028-ABE8e

   Plasmid

   #138492

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1028)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plenti-ARE-HSVTK-luc

   Plasmid

   #161789

   Purpose

   Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

   Depositor

   Hans-Guido Wendel

   Article

   Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

   Inserts

   Luciferase-T2A-HSV-TK
   mCherry P2A blasticidin
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   EF1a and Murine Gclc ARE

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CA

   Plasmid

   #20960

   Purpose

   piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKMyc

    Plasmid

    #19400

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Type

    Empty backbone

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. Talin-CS

    Plasmid

    #83377

    Purpose

    Control FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted at C-terminal o…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Cristina Montagna, Elias Spiliotis

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fibronectin-human-plasma in pMAX

    Plasmid

    #120401

    Purpose

    enables eukaryotic expression of human plasma fibronectin

    Depositor

    Inaam Nakchbandi

    Article

    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

    Insert

    Fibronectin (FN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL_U2AF1_WT_mCherry

    Plasmid

    #84017

    Purpose

    expression of U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAS-luc2

    Plasmid

    #24343

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Luciferase
    

    Tags

    5XGAL4

    Expression

    Mammalian

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 deltaN89 Beta-catenin

    Plasmid

    #19288

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-89

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only