78,024 results

1. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK-Drosha(UniProt)-FLAG

   Plasmid

   #164690

   Purpose

   Express Drosha-FLAG in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   Drosha (DROSHA Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-VSVG

   Plasmid

   #35616

   Depositor

   Arthur Nienhuis, Patrick Salmon

   Insert

   VSV G protein
   

   Expression

   Mammalian

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-YFP-rictor

   Plasmid

   #73387

   Purpose

   Expresses HA and YFP tagged Rictor

   Depositor

   Jie Chen, Taekjip Ha

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twinkle-APEX2-V5

   Plasmid

   #129705

   Purpose

   APEX2 fused to the mitochondrial nucleoid protein Twinkle

   Depositor

   Alice Ting

   Article

   Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

   Insert

   Twinkle-APEX2-V5 (TWNK, APEX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-NL1B

   Plasmid

   #15261

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Neuroligin 1 with insert B (Nlgn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205317

   Purpose

   All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISYmu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMax_ScaI_BFP

   Plasmid

   #177824

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP
   

   Expression

   Mammalian

   Mutation

   5' inserted ScaI RE cut site

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1761

   Plasmid

   #142843

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF24 (ZNF24 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-myc-Cdc42-T17N

    Plasmid

    #12973

    Depositor

    Gary Bokoch

    Insert

    Cdc42 DN (CDC42 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF-BOS-RIG-I-Myc

    Plasmid

    #215872

    Purpose

    RIG-I-Myc expression vector for overexpression

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

    Insert

    RIG-I (RIGI Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPHK2 gRNA (BRDN0001146242)

    Plasmid

    #77095

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-scFvGCN4sfGFP-VP64-GB1

    Plasmid

    #141417

    Purpose

    Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

    Depositor

    Izuho Hatada

    Article

    Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

    Insert

    scFvGCN4sfGFP-VP64-GB1
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-PLIN3

    Plasmid

    #222426

    Purpose

    To express PLIN3 with mEGFP tag in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    PLIN3 (PLIN3 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid

    #125256

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2(H134R)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a-forward

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mGreenLantern

    Plasmid

    #197928

    Purpose

    Monomer expression vector

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    mGreenLantern
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIC301

    Plasmid

    #69732

    Purpose

    Retroviral expression of Bub1

    Depositor

    Iain Cheeseman

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAXEF-Rap1-WT

    Plasmid

    #32697

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Promoter

    EF promoter

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only