80,766 results

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pMRX-IPU-FLAG-NEK9

Plasmid

#168269

Purpose

Expresses NEK9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A Q79L

Plasmid

#28046

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Tags

EGFP on N-term

Expression

Mammalian

Mutation

Q79L

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF0589

Plasmid

#142653

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXO1 (FOXO1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLIX402 PHF2

Plasmid

#91700

Purpose

inducible lentiviral expression of PHF2

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mem-seTurboID

Plasmid

#237887

Purpose

AAV vector for Cre-dependent expression of membrane-tethered seTurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

mem-seTurboID

Use

AAV and Cre/Lox

Tags

FLAG tag and GAP43 palmitoylation signal

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-NMNAT2

Plasmid

#133260

Purpose

Express FLAG-tagged NMNAT2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT2 (NMNAT2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-mStayGold

Plasmid

#227251

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDest eGFP MBNL1 42 kDa

Plasmid

#61277

Purpose

Mammalian expression of EGFP-tagged human MBNL1

Depositor

Nicolas Charlet-Berguerand

Insert

MBNL1 (MBNL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR-NES

Plasmid

#205567

Purpose

Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR-NES (RAF1 Synthetic, Human)

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag7

Plasmid

#169330

Purpose

Expression of TOMM20-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag7 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

Plasmid

#79373

Purpose

Recruits VPR to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8

Plasmid

#11817

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 (CASP8 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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OMM-Flag-APEX2-SOPP3

Plasmid

#229073

Purpose

Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PB_p300_core-dCas9-EGFP_hygro

Plasmid

#226431

Purpose

PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

Depositor

Alon Goren

Inserts

p300 core-dCas9-EGFP fusion protein
rtTA3-IRES-Hygro

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

HA tag, P2A-EGFP, and p300 core

Expression

Mammalian

Promoter

Dox inducible minimal CMV and UbC Promoter

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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REEP5-EGFP

Plasmid

#210363

Purpose

Expresses REEP5 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 Gag-imCherry

Plasmid

#208882

Purpose

codes for Gag from HIV-1 with an internal mCherry fluorescent protein

Depositor

Raphael Gaudin

Article

Diana Brychka et al bioRxiv

Insert

HIV-1 Gag

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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SFB-SLX4

Plasmid

#247854

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

SLX4 (SLX4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-TetOn-mCherry-OAZ1_FS-YFP

Plasmid

#232356

Purpose

Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

Depositor

Ankur Jain

Article

Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

Insert

OAZ1 derived polyamine sensing module (OAZ1 Human)

Use

Lentiviral

Tags

eYFP and mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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