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pcDNA3.1-Myc-14-3-3ζ

Plasmid

#48798

Purpose

expresses human myc-14-3-3ζ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAZ (YWHAZ Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-Nkx2-1/Puro

Plasmid

#31271

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

Nkx2-1* (Nkx2-1 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx1

Plasmid

#202751

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170):mNG2 (PLCD1 Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/Nuc-mutant-hogg1

Plasmid

#18710

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Expression

Mammalian

Mutation

Amino Acid 229 Arginine changed to Glutamine

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pLAS2w.Phyg-HAVCR1

Plasmid

#232669

Purpose

expresses HAVCR1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chen et al HGG Adv. 2026 May 25:100630. doi: 10.101

Insert

HAVCR1 (HAVCR1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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WT NFAT2

Plasmid

#11101

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

3xHA and GFP

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT6_H133Y

Plasmid

#53149

Purpose

expression vector for catalyitc inactive human Sirtuin6

Depositor

Katrin Chua

Article

Michishita et al Nature. 2008 Mar 27;452(7186):492-6. doi

Insert

Sirt6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H133Y, catalytic inactive

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_ISFV_G

Plasmid

#207282

Purpose

Mammalian expression of Isfahan virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ISFV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-ER-MT-P2A

Plasmid

#214892

Purpose

Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-ER-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαi1-LgB91

Plasmid

#134342

Purpose

Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαi1-LgB91 (GNAI1 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA1

Plasmid

#26677

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 1 (KPNA1 Human)

Tags

T7

Expression

Mammalian

Mutation

KPNA1 sequence contains a reported SNP at S73N

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD1

Plasmid

#153116

Purpose

Expression of Flag tagged human FOXD1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD1 (FOXD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1(R40L)-GFP

Plasmid

#51408

Purpose

Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 40 mutated to leucine

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

Plasmid

#209294

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(RNR2-Left)-MutH

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-25xUPRE-minP-EGFP

Plasmid

#159668

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xUPRE-minP-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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TMED10-bio-His

Plasmid

#51852

Purpose

Expresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TMED10 (TMED10 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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RMCE wild type DNA-PKcs

Plasmid

#233911

Purpose

Low copy number provides expression of human DNA-PKcs

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

dna-pkcs cDNA (PRKDC Human)

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5(TA)-ires-puro

Plasmid

#167823

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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