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CLYBL-Bxb1-LP-v2-TC

Plasmid

#194327

Purpose

CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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c-Fos

Plasmid

#187905

Purpose

Expresses c-Fos in transfected cells

Depositor

Jennifer Mitchell

Publication

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

cFos (FOS Human)

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Publication

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-Jun (JUN Human)

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAVS1-Bxb1-LP-TC

Plasmid

#183754

Purpose

AAVS1 targeting construct with the Bxb1 landing pad cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox257

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-Nme2ABE8e

Plasmid

#189928

Purpose

Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Publication

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nme2ABE8e

Tags

NLS

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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cytoplasmic cpVenus control

Plasmid

#186788

Purpose

Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Publication

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

cytoplasmic cpVenus control

Use

Lentiviral

Expression

Mammalian

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTS395_PhCMV-SB100

Plasmid

#169633

Purpose

Expression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven SB100

Expression

Mammalian

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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GPI-mEGFP

Plasmid

#182866

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

GPI-mEGFP

Expression

Mammalian

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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OMMGFP 1-10

Plasmid

#164119

Purpose

Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

Depositor

Marisa Brini, Tito Calì

Publication

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OMMGFP 1-10

Expression

Mammalian

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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H2B-mGreenLantern

Plasmid

#164464

Purpose

Localization of mGreenLantern fluorescent protein to histone 2B

Depositor

Gregory Petsko

Publication

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mGreenLantern

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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eMagAF-EGFP-PM

Plasmid

#162247

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Publication

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eMagAF-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CTCF-mAC donor (Hygro)

Plasmid

#140646

Purpose

CTCF tagging with mAID-clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiGuide-hygro

Plasmid

#160090

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLEX307-SARS-CoV-2-3CL

Plasmid

#160278

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Gαq*-BERKY3

Plasmid

#158217

Purpose

BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-OMP25

Plasmid

#141150

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OMP25 (Synj2bp Rat)

Tags

AcGFP

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125691

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Publication

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-GGamma9-GFP2

Plasmid

#140991

Purpose

Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GGamma9-GFP2 (GNGT2 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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