78,128 results

1. pTRE-Medium-FT

   Plasmid

   #31914

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Medium-FT
   

   Expression

   Mammalian

   Mutation

   M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Sept. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-Mem

   Plasmid

   #55779

   Purpose

   This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   mCherry-Mem
   

   Tags

   The N-terminal 20 residues of neuromodulin are fu…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2193

   Plasmid

   #141964

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICE-HA-MRE11-WT

   Plasmid

   #82033

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. P210 pcDNA3

   Plasmid

   #27481

   Depositor

   Warren Pear

   Article

   He et al Blood. 2002 Apr 15;99(8):2957-68.

   Insert

   bcr/abl (ABL1, BCR Human)
   

   Expression

   Mammalian

   Mutation

   Contains the complete bcr/abl fusion

   Promoter

   CMV

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE3G-Bi-β-chain

   Plasmid

   #133731

   Purpose

   Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   b-Chain_gePSI
   ZsGreen1
   

   Expression

   Mammalian

   Mutation

   Only one part of the gePSI-system (startcodon + a…

   Promoter

   pTRE3G-Bi

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-neo FlucDM EGFP

   Plasmid

   #90172

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Expression

   Mammalian

   Mutation

   R188Q, R261Q in FLuc

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. NPM1_mNeonGreen_Donor

   Plasmid

   #178093

   Purpose

   HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1-mNeonGreen HDR donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0120

   Plasmid

   #142539

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ELMSAN1 (MIDEAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0095

    Plasmid

    #142145

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZEB2 (ZEB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mMaroon1

    Plasmid

    #83840

    Purpose

    Expresses mMaroon1

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid

    #29752

    Depositor

    Edward McCabe

    Insert

    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    

    Tags

    EGFP, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; Reporter is nuclear localized EGFP;…

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. BFP-Rab5CA (Q79L)

    Plasmid

    #205705

    Purpose

    Mammalian expression of BFP-tagged mutant Rab5CA

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    RAB5A (RAB5A Human)
    

    Expression

    Mammalian

    Mutation

    Q79L

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFlag-CMV2-Brd4 del ET

    Plasmid

    #21938

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-1362 only, ET domain deleted

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV, lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. HIR WT

    Plasmid

    #24049

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HA-USP15

    Plasmid

    #22570

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP15 (USP15 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-MTS-jAspSnFR3-mRuby3

    Plasmid

    #203492

    Purpose

    Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3-mRuby3
    

    Use

    Lentiviral

    Tags

    His-tag, Mitochondrial localization signal, and m…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EFSp-GFP-YAP

    Plasmid

    #174168

    Purpose

    Lentiviral vector to constitutively express YAP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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