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SMN2E7-Fluc-Nluc-reporter

Plasmid

#221005

Purpose

Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

Fluc-SMN2E7-STOP-Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP Cortactin

Plasmid

#50728

Purpose

mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Insert

Cortactin (CTTN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

Plasmid

#239972

Purpose

Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-APEX2-nDEST

Plasmid

#214992

Purpose

Gateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tag

Depositor

Wade Harper

Type

Empty backbone

Use

Lentiviral

Tags

Flag-APEX2

Expression

Mammalian

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ch-Tog

Plasmid

#28290

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Expression

Bacterial and Mammalian

Mutation

K19R, E118G

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Farnesyl-5

Plasmid

#55295

Purpose

Localization: Plasma Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Farnesyl (HRAS Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3-STAT3-Y705F

Plasmid

#74434

Purpose

Expression of STAT3-Y705F

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

Flag

Expression

Mammalian

Mutation

Mutations E16K, Y705F

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-beta2AR

Plasmid

#106063

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-beta2AR

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-KDEL

Plasmid

#226568

Purpose

Encodes mNeonGreen with KDEL ER lumenal target sequence

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

KDEL (ER lumenal target sequence)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Blast myc

Plasmid

#10674

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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HaloTag-dSpCas9

Plasmid

#238946

Purpose

N-terminal fusion of HaloTag to dSpCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

HaloTag

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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hCathepsin B

Plasmid

#11249

Purpose

Mammalian expression of human cathepsin B

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin B (CTSB Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3 1kb prom. CD274

Plasmid

#107002

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-NET-myc

Plasmid

#107458

Purpose

Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Norepinephrine Transporter (NET) (SLC6A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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