75,750 results

1. pNLF1-C_hCOL4A5-Nluc

   Plasmid

   #198113

   Purpose

   Express hCOL4A5 in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Tags

   Nluc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-NFAT1(4-460)-GFP

   Plasmid

   #11107

   Purpose

   Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Use

   Tags

   3x HA and GFP

   Expression

   Mammalian

   Mutation

   Contains amino acids 4-460 of NFAT1; L78P compare…

   Promoter

   CMV

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-U6

   Plasmid

   #120358

   Purpose

   piggyBac transposon vector for sgRNA expression.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.1 puro shRNA beta-catenin

   Plasmid

   #18803

   Purpose

   3rd gen lentiviral vector for knocking down beta-catenin gene expression

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   b-catenin shRNA (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pActin-miRFP670nano3

   Plasmid

   #184665

   Purpose

   Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-human beta-actin
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-C-mStayGold-EFS-Blast

   Plasmid

   #227257

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-3xFLAG-BioID2-MCS

   Plasmid

   #186811

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-BioID2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXs-mGCN2-FLAG-IP

   Plasmid

   #101794

   Purpose

   Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

   Depositor

   Seiichi Oyadomari

   Article

   Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

   Insert

   Gcn2 (Eif2ak4 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-TLR5-CFP

   Plasmid

   #13019

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 5 (TLR5 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Spectrin-C-cpstFRET

    Plasmid

    #61111

    Purpose

    Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

    Depositor

    Fred Sachs

    Article

    Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

    Insert

    spectrin-cpstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROPseq-Guide-Zeo

    Plasmid

    #127173

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-Calnexin-N-14

    Plasmid

    #55005

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXG289

    Plasmid

    #141283

    Purpose

    OE of DELE1

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    DELE1 (DELE1 Human)
    

    Use

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-VSVG

    Plasmid

    #35616

    Depositor

    Patrick Salmon, Arthur Nienhuis

    Insert

    VSV G protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX307 MCL1

    Plasmid

    #117726

    Purpose

    Open reading frame vector encoding MCL1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    MCL1L (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CROPseq-multi-EBFP2-NLS (pRW1447)

    Plasmid

    #216219

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR_zeo_sgTP53

    Plasmid

    #183180

    Purpose

    pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgTP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. beta Synuclein-VC

    Plasmid

    #89484

    Purpose

    expresses human beta Synuclein fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Use

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-ShadowG

    Plasmid

    #104620

    Purpose

    Expresses Shadow G in mammalian cell

    Depositor

    Hideji Murakoshi

    Article

    Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

    Insert

    ShadowG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge