78,128 results

1. Flag-M4-AR

   Plasmid

   #171240

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor (AR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. BFP-Rab5CA (Q79L)

   Plasmid

   #205705

   Purpose

   Mammalian expression of BFP-tagged mutant Rab5CA

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   RAB5A (RAB5A Human)
   

   Expression

   Mammalian

   Mutation

   Q79L

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 gfaABC1D-LifeAct-GFP

   Plasmid

   #190199

   Purpose

   Expresses the small actin binding peptide, LifeAct, with a GFP reporter

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   LifeAct-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1-REX-YFP

   Plasmid

   #48247

   Purpose

   Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

   Insert

   REX-YFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlag-CMV2-Brd4 del ET

   Plasmid

   #21938

   Depositor

   Eric Verdin

   Article

   Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-1362 only, ET domain deleted

   Available Since

   Sept. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pCI-M2

   Plasmid

   #44170

   Depositor

   John Olsen

   Article

   Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

   Insert

   Influenza A virus M2 matrix gene
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-gRNA-Puro

   Plasmid

   #121121

   Purpose

   Expression of a gRNA together with a Puromycin resistance

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. tdTomato-C1

    Plasmid

    #54653

    Purpose

    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. NPY5R-Tango

    Plasmid

    #66456

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NPY5R (NPY5R Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid

    #145780

    Purpose

    Over expression of SARS-CoV-2 S (spike) in mammalian cell line

    Depositor

    Zhaohui Qian

    Article

    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

    Insert

    SARS-CoV-2 S (S SARS CoV 2)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Codon optimized and C-terminal 19 amino acids del…

    Promoter

    CMV

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5B-Flag-Smad3

    Plasmid

    #11742

    Depositor

    Jeff Wrana

    Article

    Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

    Insert

    SMAD3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. mEmerald-CD36-C-10

    Plasmid

    #54030

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. TFORF0120

    Plasmid

    #142539

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELMSAN1 (MIDEAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HA-UBA6

    Plasmid

    #136995

    Purpose

    Expresses human UBA6 in mammalian cells

    Depositor

    Marcus Groettrup

    Article

    Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

    Insert

    UBA6 (UBA6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-SARS-CoV-2-S (Wuhan)

    Plasmid

    #215812

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-flag-GFP-Ecad

    Plasmid

    #205188

    Purpose

    This vector encodes of a synCAM (Ecad) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Ecad and GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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