80,093 results

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_UAS-tTS-P2A-GFP_PGK-BFP

Plasmid

#133808

Purpose

inducible expression of tTS and GFP with BFP marker in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

tTS-P2A-EGFP-PGK-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1916

Plasmid

#144317

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX2 (RUNX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-GFP

Plasmid

#28304

Purpose

Cre-dependent AAV-mediated expression of GFP under the CAG promoter

Depositor

Edward Boyden

Insert

GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro-H2BC11-MMEJ

Plasmid

#227333

Purpose

MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_GATA4

Plasmid

#167758

Purpose

Expresses GATA4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

GATA4 (GATA4 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Article

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLAI-Env

Plasmid

#133996

Purpose

Env gene from HIV-1 LAI in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-TdTomato

Plasmid

#191203

Purpose

FlpO dependent TdTomato expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

TdTomato

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

Plasmid

#52505

Purpose

mammalian expression vector of 53BP1 28A mutant

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 28A (TP53BP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

All 28 SQ/TQ motifs in N-term of 53BP1 have been …

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-N-Myc_1-560 USP7

Plasmid

#131244

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 1 - 560. This truncati…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-C7orf59

Plasmid

#42332

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v8-10 (fl)

Plasmid

#137823

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-BACH1

Plasmid

#232269

Purpose

Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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HA-APP-mGFP

Plasmid

#196696

Purpose

Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

HA and mGFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-CAV

Plasmid

#168303

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the caveolae

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-CAV

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLOX-gfp-iresTK

Plasmid

#12243

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

GFP, HSV1-TK

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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TFORF2904

Plasmid

#143388

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DACH1 (DACH1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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