80,093 results

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pHR-SIN-BX SpyCatcher003-hCD52

Plasmid

#210565

Purpose

Expresses Spycatcher003 on the cell surface using human CD52 hinge

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Spycatcher003 fused to human CD52 hinge

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP 150TAG

Plasmid

#197570

Purpose

Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
Pyl-tRNA (4 copies)

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag SRPK2

Plasmid

#20525

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

SRPK2 (SRPK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
mCherry-OSBP

Plasmid

#232871

Purpose

Expresses OSBP in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

mCherry-OSBP (OSBP Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10

Plasmid

#180348

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SSTR2-Tango

Plasmid

#66503

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

SSTR2 (SSTR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-tTA

Plasmid

#127091

Purpose

An AAV genome with Cre-dependent expression of tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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SIRT5 Flag

Plasmid

#13816

Purpose

Mammalian expression of human SIRT5 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT5 (SIRT5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr261-EGFP

Plasmid

#58471

Purpose

Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr261 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-NES-eNAT10

Plasmid

#238299

Purpose

Expresses cytoplasmic version of the programmable RNA acetylation system.

Depositor

Won Do Heo

Article

Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

Insert

dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)

Use

CRISPR

Tags

HIV NES (C terminal of dPspCas13b)

Expression

Mammalian

Mutation

H133A for catalytically inactive mutant, deletion…

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Halo-CAAX

Plasmid

#249173

Purpose

Mammalian expression vector of CAAX motif (plasma membrane marker) fused with Halo-tag

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

CAAX (HRAS Human)

Tags

Halo

Expression

Mammalian

Mutation

Only 10 amino acids ending with CVLS (CAAX motif)

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-IRES-GFP

Plasmid

#65433

Purpose

Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-TRAF1

Plasmid

#111199

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

TRAF1 (TRAF1 Human)

Tags

EGFP-FLAG

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Six1

Plasmid

#49263

Purpose

retroviral expression of human Six1 and GFP

Depositor

Heide Ford

Insert

Six1 (SIX1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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IMS-APEX2

Plasmid

#79058

Purpose

Expresses APEX in the mitochondrial intermembrane space.

Depositor

Alice Ting

Article

Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

Insert

APEX

Tags

V5

Expression

Bacterial and Mammalian

Promoter

T7 promoter

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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